by Norman Wai-sing Lo. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 145-160). / Chapter Chapter 1 --- Introduction --- p.1 / Chapter A. --- Classification of Salmonella --- p.3 / Chapter B. --- Enteric fever --- p.4 / Chapter C. --- Chloramphenicol and multiple resistance in S. Typhi --- p.7 / Chapter D. --- Resistance plasmids in the typhoid bacillus --- p.11 / Chapter E. --- In vivo acquisition of resistance plasmids by S. Typhi --- p.18 / Chapter F. --- Worldwide distribution of typhoid fever --- p.23 / Chapter G. --- Epidemiology of typhoid fever in Hong Kong --- p.27 / Chapter H. --- Principles of methods used in the epidemiological typing of S. Typhi --- p.29 / Chapter 1. --- Phage typing --- p.29 / Chapter 2. --- Antibiotics sensitivity pattern --- p.32 / Chapter 3. --- Plasmid analyses --- p.32 / Chapter a. --- Genetic methods --- p.33 / Chapter i. --- Phenotypic expression --- p.33 / Chapter ii. --- Transferability of plasmids --- p.33 / Chapter iii. --- Incompatibility --- p.34 / Chapter b. --- Molecular methods --- p.34 / Chapter i. --- Estimation of molecular size by agarose gel electrophoresis --- p.34 / Chapter ii. --- Plasmid fingerprinting --- p.35 / Chapter iii. --- Localization of resistance genes by DNA-DNA hybridization --- p.35 / Chapter 4. --- Ribotyping --- p.36 / Chapter 5. --- Total DNA fingerprinting --- p.37 / Chapter 6. --- Chromosomal insertion sequence IS200 profile analysis --- p.38 / Chapter 7. --- Others --- p.39 / Chapter a. --- Multilocus enzyme electrophoresis --- p.39 / Chapter b. --- Phenotype of lipopolysaccharide (LPS) --- p.39 / Chapter c. --- Envelope protein profiles and immunoblotting --- p.40 / Chapter I. --- Epidemiological typing of S. Typhi --- p.40 / Chapter J. --- Objectives --- p.46 / Chapter Chapter 2 --- Materials and Methods --- p.48 / Chapter A. --- Materials --- p.48 / Chapter I. --- Bacterial strains --- p.48 / Chapter II. --- Materials --- p.51 / Chapter B. --- Methods --- p.56 / Chapter I. --- Sensitivity testing --- p.56 / Chapter II. --- Characterization of plasmids --- p.58 / Chapter 1. --- Genetic studies --- p.58 / Chapter a. --- Transferability of resistance plasmids --- p.58 / Chapter b. --- Mobilization of resistances --- p.59 / Chapter c. --- Incompatibility grouping --- p.59 / Chapter 2. --- Molecular studies --- p.60 / Chapter a. --- Plasmid profile analysis --- p.60 / Chapter i. --- Plasmid extraction --- p.60 / Chapter ii. --- Agarose gel electrophoresis --- p.61 / Chapter b. --- Molecular characterization of plasmid --- p.61 / Chapter i. --- Extraction of purified plasmid DNA --- p.62 / Chapter ii. --- Restriction endonuclease digestion of plasmid DNA --- p.63 / Chapter III. --- Characterization β-lactamases --- p.63 / Chapter 1. --- Extraction of β-lactamases --- p.63 / Chapter 2. --- Determination of isoelectric points (pIs) --- p.64 / Chapter IV. --- Localization of resistance genes --- p.64 / Chapter 1. --- TEM-1 and TEM-2 genes --- p.65 / Chapter a. --- Oligonucleotide probes --- p.65 / Chapter b. --- Total DNA preparation --- p.66 / Chapter c. --- DNA-DNA-hybridization --- p.66 / Chapter i. --- Transfer of DNA to membrane filters --- p.66 / Chapter ii. --- Labelling of oligonucleotide probes (TEM-1 and TEM-2) --- p.67 / Chapter iii. --- Hybridization --- p.68 / Chapter 2. --- Chloramphenicol- and tetracycline-resistance genes --- p.69 / Chapter a. --- Preparation of probes --- p.69 / Chapter b. --- Hybridization --- p.73 / Chapter i. --- Transfer of DNA to membrane filters --- p.73 / Chapter ii. --- Labelling of chloramphenicol- and tetracycline- resistance probes --- p.73 / Chapter V. --- Epidemiological typing --- p.75 / Chapter 1. --- Ribotyping --- p.75 / Chapter 2. --- DNA fingerprinting --- p.77 / Chapter C. --- Plan for achieving objectives --- p.77 / Chapter Chapter 3 --- Results --- p.79 / Chapter A. --- Antimicrobial susceptibilities --- p.79 / Chapter B. --- Characterization of resistance plasmids --- p.83 / Chapter C. --- β-lactamases produced by ampicillin-resistant S. Typhi --- p.85 / Chapter D. --- Localization of resistance genes --- p.85 / Chapter E. --- Plasmid profile analysis --- p.87 / Chapter F. --- Plasmid fingerprinting --- p.90 / Chapter G. --- Ribotyping --- p.93 / Chapter H. --- Chromosomal DNA fingerprinting --- p.107 / Chapter I. --- "Correlation of PstI, ClaI and KpnI ribotypes and NarI, EcoRV and MluI chromosomal types" --- p.124 / Chapter J. --- Correlation of PstI ribotypes and NarI chromosomal types --- p.126 / Chapter K. --- Epidemiology of S. Typhi in Hong Kong --- p.129 / Chapter Chapter 4 --- Discussion --- p.131 / Chapter A. --- Antimicrobial susceptibilities --- p.131 / Chapter B. --- Characterization of resistances --- p.132 / Chapter C. --- Plasmid profile analysis --- p.135 / Chapter D. --- Epidemiological analysis of S. Typhi --- p.136 / Chapter E. --- Area for future research --- p.143 / References --- p.145 / Appendix --- p.161 / Chapter A. --- Buffer and Stock solutions --- p.161 / Chapter B. --- Epidemiological information of S. Typhi isolates --- p.167
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_320701 |
| Date | January 1994 |
| Contributors | Lo, Norman Wai-sing., Chinese University of Hong Kong Graduate School. Division of Pathological Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 178 leaves : ill. (some mounted) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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