Leung Yiu Fai Ivan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 41-46). / Acknowledgements --- p.ii / Table of contents --- p.iii / List of tables --- p.vi / List of figures --- p.vii / List of Abbreviations --- p.xii / Abstract --- p.xiii / Chapter I. --- INTRODUCTION --- p.1 / Chapter A. --- Current knowledge on carotenoids --- p.1 / Chapter 1. --- Chemistry --- p.1 / Chapter 2. --- Occurrence --- p.3 / Chapter 3. --- Metabolism --- p.6 / Chapter 4. --- Biological function --- p.8 / Chapter B. --- Statement of the problem --- p.10 / Chapter II. --- MATERIAL AND METHODS --- p.11 / Chapter A. --- Materials --- p.11 / Chapter 1. --- Animals --- p.11 / Chapter 2. --- Human samples --- p.11 / Chapter 3. --- Chemicals --- p.12 / Chapter 4. --- HPLC Apparatus --- p.12 / Chapter B. --- Methods --- p.14 / Chapter 1. --- Animal specimens --- p.14 / Chapter (a) --- Carotenoid supplement --- p.14 / Chapter (b) --- Tissue preparation --- p.14 / Chapter 2. --- Human specimens --- p.15 / Chapter 3. --- Extraction of lipid component --- p.15 / Chapter (a) --- Retina --- p.15 / Chapter (b) --- Serum or subretinal fluid --- p.16 / Chapter (c) --- Liver --- p.16 / Chapter 4. --- Analytical methods --- p.17 / Chapter (a) --- Isocratic elution --- p.17 / Chapter (b) --- Gradient elution --- p.17 / Chapter III. --- RESULTS --- p.18 / Chapter A. --- Selection of chromatographic method for carotenoid analysis --- p.18 / Chapter 1. --- Effect of dioxane concentration on the retention time of carotenoids --- p.18 / Chapter (a) --- Gradient elution --- p.18 / Chapter (b) --- Isocratic elution --- p.18 / Chapter 2. --- Chromatograms of carotenoids and retinoids of a selected method for routine analysis --- p.19 / Chapter (a) --- Carotenoids --- p.20 / Chapter (b) --- Retinoids --- p.21 / Chapter B. --- Application of the selected method to study carotenoid in human and rats --- p.22 / Chapter 1. --- Study in human serum and subretinal fluid --- p.22 / Chapter (a) --- Serum --- p.22 / Chapter (b) --- Subretinal fluid --- p.22 / Chapter 2. --- Study in rats tissues --- p.24 / Chapter (a) --- Liver --- p.24 / Chapter (b) --- Serum --- p.26 / Chapter (c) --- Retina --- p.28 / Chapter C. --- Influence of dietary carotenoids on retinol concentration in rats --- p.31 / Chapter 1 --- Serum --- p.31 / Chapter 2 --- Retina --- p.31 / Chapter IV. --- DISCUSSION --- p.32 / Chapter A. --- Chromatographic analysis of carotenoids and retinoids --- p.32 / Chapter B. --- Carotenoid study in human and rats --- p.33 / Chapter 1. --- Carotenoids in human tissues --- p.33 / Chapter (a) --- Serum --- p.33 / Chapter (b) --- Subretinal fluid --- p.34 / Chapter 2. --- Dietary supplement of carotenoids to rats --- p.35 / Chapter (a) --- Choice of animal and types of carotenoids --- p.35 / Chapter (b) --- Carotenoids uptake into rats tissues --- p.36 / Chapter (c) --- Effect of dietary carotenoids on retinol concentration in rat tissues --- p.38 / Chapter V. --- CONCLUSION --- p.39 / Chapter VI. --- REFERENCES --- p.41 / Chapter VII. --- TABLES --- p.47 / Chapter VIII. --- FIGURES --- p.56
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322022 |
| Date | January 1997 |
| Contributors | Leung, Yiu Fai Ivan., Chinese University of Hong Kong Graduate School. Division of Surgical Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 105 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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