by Chan, Kim Hung. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 76-89). / Abstract also in Chinese. / Chapter 1. --- INTRODUCION --- p.1 / Chapter 1.1 --- amino acid analysis by high performance liquid chromatography --- p.1 / Chapter 1.1.1 --- History and Development --- p.1 / Chapter 1.1.2 --- Separation mechanism --- p.2 / Chapter 1.1.3 --- Derivatization --- p.4 / Chapter 1.1.4 --- Dqproteinization --- p.8 / Chapter 1.1.5 --- Ion-exchange or Reversed-phase HPLC --- p.9 / Chapter 1.2 --- amino acid pattern in cancer patient --- p.11 / Chapter 1.2.1 --- Cancer cachexia --- p.11 / Chapter 1.2.2 --- Causes of cancer cachexia --- p.11 / Chapter 1.2.3 --- Cytokines --- p.12 / Chapter 1.2.4 --- Metabolic Alteration in cancer cachexia --- p.13 / Chapter 1.2.5 --- Amino Acid Studies --- p.14 / Chapter 1.3 --- methodology chosen --- p.19 / Chapter 1.4 --- patient sample chosen --- p.21 / Chapter 2. --- OBJECTIVES --- p.22 / Chapter 3. --- MATERIALS AND METHOD --- p.23 / Chapter 3.1 --- apparatus --- p.23 / Chapter 3.1.1 --- HPLC System --- p.23 / Chapter 3.1.2 --- Column --- p.23 / Chapter 3.1.3 --- Detector --- p.23 / Chapter 3.1.4 --- ChemStation --- p.24 / Chapter 3.2 --- reagents --- p.24 / Chapter 3.2.1 --- Reagent and Chemical source --- p.24 / Chapter 3.2.2 --- Mobile phase --- p.24 / Chapter 3.2.3 --- Derivatization Reagent --- p.25 / Chapter 3.2.4 --- Standard preparation --- p.26 / Chapter 3.2.5 --- Internal standard --- p.28 / Chapter 3.3 --- sample preparation --- p.28 / Chapter 3.4 --- chromatographic conditions --- p.29 / Chapter 3.4.1 --- Column Temperature --- p.29 / Chapter 3.4.2 --- Injector Program --- p.29 / Chapter 3.4.3 --- Time Table for gradient elution and flow program --- p.32 / Chapter 3.5.1 --- OP A and sample Ratio and Volume --- p.32 / Chapter 3.5.2 --- Derivatization Concentration --- p.33 / Chapter 3.5.3 --- Derivatization time --- p.33 / Chapter 3 6 --- analytical performance --- p.34 / Chapter 3.6.1 --- Linearity testing --- p.34 / Chapter 3.6.2 --- Recovery studies --- p.34 / Chapter 3.6.3 --- Precision --- p.34 / Chapter 3.6.4 --- Sample storage --- p.35 / Chapter 3.7 --- clinical sample studies --- p.35 / Chapter 3.8 --- statistical analysis --- p.36 / Chapter 4 --- RESULT --- p.37 / Chapter 4.1 --- chromatographic separation --- p.37 / Chapter 4.2 --- optimization --- p.40 / Chapter 4.2.1 --- OPA and sample Ratio and Volume --- p.40 / Chapter 4.2.2 --- Derivatization time --- p.43 / Chapter 4.2.3 --- OPA Concentration --- p.43 / Chapter 4.3 --- analytical performance --- p.46 / Chapter 4.3.1 --- Linearity --- p.46 / Chapter 4.3.2 --- Recovery studies --- p.46 / Chapter 4.3.3 --- Precision Studies --- p.50 / Chapter 4.3.4 --- Sample storage studies --- p.53 / Chapter 4.4 --- clinical sample study --- p.55 / Chapter 5. --- DISCUSSION --- p.64 / Chapter 5.1 --- analytical --- p.64 / Chapter 5.2 --- clinical --- p.71 / Chapter 5.2.1 --- Normal controls --- p.71 / Chapter 5.2.2 --- Colorectal Cancer --- p.71 / Chapter 5.2.3 --- Lung Cancer --- p.72 / Chapter 5.2.4 --- Nasopharyngeal Cancer --- p.73 / Chapter 5.2.5 --- Summary --- p.74 / Chapter 6. --- CONCLUSION --- p.75 / Chapter 7. --- REFERENCES --- p.75
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322506 |
| Date | January 1998 |
| Contributors | Chan, Kim Hung., Chinese University of Hong Kong Graduate School. Division of Chemical Pathology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, vi, 89 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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