Anita K.L. Yiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 93-112). / Acknowledgement --- p.i / List of Abbreviations --- p.ii / Abstract --- p.iv / Table of Contents --- p.vi / Chapter CHAPTER 1. --- Introduction / Chapter 1.1 --- The Renin-Angiotensin System (RAS) --- p.1 / Chapter 1.2 --- Physiology and Pathophysiology of Angiotensin --- p.3 / Chapter 1.3 --- Angiotensin Receptors / Chapter 1.3.1 --- Heterogeneity among Angiotensin Receptors --- p.10 / Chapter 1.3.2 --- Differential Distribution of Subtypes --- p.13 / Chapter 1.3.3 --- Molecular Structure of Subtypes --- p.15 / Chapter 1.3.4 --- Signal Transduction Mechanism --- p.20 / Chapter 1.3.5 --- Physiological Functional Correlates --- p.21 / Chapter 1.4 --- Aim of Study --- p.23 / Chapter CHAPTER 2. --- Preparation of Polyclonal Antibodies Against Angiotensin Receptors / Chapter 2.1 --- Introduction --- p.25 / Chapter 2.2 --- Methods / Chapter 2.2.1 --- Preparation of antisera / Chapter 2.2.1.1 --- Preparation of peptide conjugates --- p.25 / Chapter 2.2.1.2 --- Protein determination --- p.27 / Chapter 2.2.1.3 --- Immunization of rabbits with peptide conjugates --- p.27 / Chapter 2.2.1.4 --- Collection of rabbit sera --- p.28 / Chapter 2.2.1.5 --- Affinity purification of antisera --- p.28 / Chapter 2.2.2 --- Enzyme-linked immunosorbent assay (ELISA) / Chapter 2.2.2.1 --- Titer determination --- p.29 / Chapter 2.2.2.2 --- Specificity determination --- p.30 / Chapter 2.3 --- Results / Chapter 2.3.1 --- Preparation of antisera --- p.30 / Chapter 2.3.2 --- Affinity purification of antisera --- p.30 / Chapter 2.3.3 --- ELISA / Chapter 2.3.3.1 --- Titer determination --- p.31 / Chapter 2.3.3.1.1 --- Thy-AT1 antiserum --- p.31 / Chapter 2.3.3.1.2 --- Thy-AT2 antiserum --- p.32 / Chapter 2.3.3.2 --- Specificity determination --- p.32 / Chapter 2.3.3.2.1 --- Thy-AT1 antibodies --- p.32 / Chapter 2.3.3.2.2 --- Thy-AT2 antibodies --- p.49 / Chapter 2.4 --- Discussions --- p.49 / Chapter CHAPTER 3. --- Application of Thy-AT1 Antiserumin Western Blot / Chapter 3.1 --- Introduction --- p.52 / Chapter 3.2 --- Methods / Chapter 3.2.1 --- Preparation of protein samples --- p.52 / Chapter 3.2.2 --- Protein determination --- p.53 / Chapter 3.2.3 --- SDS-PAGE --- p.53 / Chapter 3.2.3 --- Western blot --- p.54 / Chapter 3.2.5 --- Immunoblotting --- p.54 / Chapter 3.3 --- Results --- p.55 / Chapter 3.4 --- Discussions --- p.58 / Chapter CHAPTER 4. --- Evaluation of Pancreatic Response to Angiotensin II / Chapter 4.1 --- Introduction --- p.61 / Chapter 4.2 --- Methods / Chapter 4.2.1 --- Perfusion of pancreas --- p.62 / Chapter 4.2.2 --- Assay of amylase activity --- p.64 / Chapter 4.2.3. --- Calculations --- p.64 / Chapter 4.3 --- Results --- p.65 / Chapter 4.4 --- Discussions --- p.65 / Chapter CHAPTER 5. --- Application of Purified Thy-AT2 Antibodies in immunohistochemical studies / Chapter 5.1 --- Introduction --- p.74 / Chapter 5.2 --- Methods / Chapter 5.2.1 --- Preparation of adrenal sections --- p.75 / Chapter 5.2.2 --- Light-microscopic immunohistochemical study --- p.76 / Chapter 5.3 --- Results / Chapter 5.4 --- Discussions / Chapter CHAPTER 6. --- General Discussions --- p.84 / References --- p.93 / Appendix / Chapter A. --- Materials --- p.113 / Chapter B. --- Buffer Compositions --- p.121
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322586 |
| Date | January 1996 |
| Contributors | Yiu, Anita K. L., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, viii, 124 leaves : ill. (some mounted) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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