by Chong Yin Yue. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 146-161). / Abstracts in English and Chinese. / Acknowledgments --- p.i / Abstract (English/Chinese) --- p.ii / Contents --- p.vi / List of Tables --- p.ix / List of Figures --- p.xi / Chapter I. --- INTRODUCTION --- p.1 / Chapter I.1. --- Central Nervous System Tumors --- p.1 / Chapter I.2. --- Histopathological Classification of Gliomas --- p.4 / Chapter I.2.1. --- Astrocytic Tumors --- p.4 / Chapter I.2.1.1. --- Diffuse Astrocytomas --- p.4 / Chapter I.2.1.1.1. --- Low Grade Diffuse Astrocytomas --- p.5 / Chapter I.2.1.1.2. --- Anaplastic Astrocytomas --- p.5 / Chapter I.2.1.1.3. --- Glioblastomas --- p.6 / Chapter I.2.1.2. --- Pilocytic Astrocytomas --- p.9 / Chapter I.2.1.3. --- Pleomorphic Xanthoastrocytomas --- p.10 / Chapter I.2.2. --- Non-Astrocytic Tumors --- p.10 / Chapter I.2.2.1. --- Oligodendroglial Tumors --- p.10 / Chapter I.2.2.2. --- Ependymal Tumors --- p.16 / Chapter I.2.2.3. --- Dysembryoplastic Neuroepithelial Tumors --- p.20 / Chapter I.3. --- Molecular Genetics in Gliomas --- p.21 / Chapter I.4. --- Telomeres and Telomerase --- p.29 / Chapter I.4.1. --- History --- p.29 / Chapter I.4.2. --- Telomeres --- p.30 / Chapter I.4.3. --- End Replication Problem --- p.32 / Chapter I.4.4. --- Telomerase --- p.34 / Chapter I.4.5. --- Telomerase Components --- p.36 / Chapter I.4.5.1. --- RNA Component --- p.36 / Chapter I.4.5.2. --- Protein Component --- p.39 / Chapter I.4.5.3. --- Catalytic Subunit --- p.40 / Chapter I.4.5.4. --- Telomeric Repeat Binding Factor 1 --- p.41 / Chapter I.4.6. --- Cellular Immortality and Aging --- p.42 / Chapter I.4.7. --- Detection of Telomerase Activity --- p.44 / Chapter I.4.7.1. --- Telomeric Repeat Amplification Protocol Assay --- p.44 / Chapter I.4.7.2. --- Other Detection Methods --- p.45 / Chapter I.4.8. --- An Overview of Telomerase Activity --- p.48 / Chapter I.4.9. --- Alternative Lengthening of Telomeres --- p.50 / Chapter I.4.10. --- Telomeres Suppressor Genes --- p.51 / Chapter I.4.11. --- Telomerase and pl6/pRb Pathway --- p.52 / Chapter I.5. --- Telomeres and Telomerase Studies in Brain Tumors --- p.54 / Chapter I.5.1. --- Telomerase Activity in Brain Tumors --- p.54 / Chapter I.5.2. --- Telomere Length in Brain Tumors --- p.57 / Chapter I.5.3. --- Telomerase RNA Component Expression in Brain Tumors --- p.57 / Chapter II. --- OBJECTIVES of STUDY --- p.59 / Chapter III. --- MATERIALS AND METHODS --- p.61 / Chapter III.l. --- Telomerase Activity Study --- p.61 / Chapter III.1.1. --- Specimens --- p.61 / Chapter III.l.2. --- Telomerase Extraction --- p.63 / Chapter III.1.3. --- Protein Concentration Measurement --- p.63 / Chapter III.1.4. --- RNase-treated Samples --- p.64 / Chapter III.l.5. --- TRAP Assay --- p.64 / Chapter III.1.5.1. --- TS Primer Labelling --- p.65 / Chapter III.1.5.2. --- TS Primer Extension And PCR Amplification --- p.65 / Chapter III. 1.6. --- Non-Denaturing Polyacrylamide Gel Electrophoresis --- p.66 / Chapter III.1.7. --- Data Analysis --- p.67 / Chapter III.1.8. --- Statistical Analysis --- p.67 / Chapter III.2. --- Telomere Length Study --- p.68 / Chapter III.2.1. --- Specimens --- p.68 / Chapter III.2.2. --- DNA Extraction --- p.70 / Chapter III.2.3. --- DNA Concentration Measurement --- p.71 / Chapter III.2.4. --- Analysis of Telomere Length by Southern Hybridization --- p.71 / Chapter III.2.4.1. --- DNA Digestion --- p.73 / Chapter III.2.4.2. --- Southern Blotting --- p.73 / Chapter III.2.5. --- Data Analysis --- p.76 / Chapter III.2.6. --- Statistical Analysis --- p.77 / Chapter III.3. --- hTERT and hTEP1 mRNA Expression Study --- p.78 / Chapter III.3.1. --- Specimens --- p.78 / Chapter III.3.2. --- RNA Extraction and DNase Treatment --- p.78 / Chapter III.3.3. --- Reverse Transcription-Polymerase Chain Reaction --- p.80 / Chapter III.3.3.1. --- Primer Design --- p.81 / Chapter III.3.3.2. --- Standard Protocol --- p.81 / Chapter III.3.4. --- Statistical Analysis --- p.84 / Chapter III.4. --- p16 and pRb Immunostaining --- p.85 / Chapter III.4.1. --- Specimens --- p.85 / Chapter III.4.2. --- Slide Preparation --- p.85 / Chapter III.4.3. --- Immunohistochemistry --- p.87 / Chapter III.4.4. --- Data Analysis --- p.88 / Chapter III.4.5. --- Statistical Analysis --- p.89 / Chapter IV. --- RESULTS --- p.90 / Chapter IV.1. --- Telomerase Activity Study --- p.90 / Chapter IV.2. --- Telomere Length Study --- p.96 / Chapter IV.3. --- hTERT and hTEPl mRNA Expression Study --- p.104 / Chapter IV.4. --- p16 and pRb Protein Expression --- p.109 / Chapter V. --- DISCUSSION --- p.115 / Chapter V. 1. --- Telomerase Activity in Gliomas --- p.115 / Chapter V.2. --- Telomere Length in Oligodendroglial and Ependymal Tumors --- p.122 / Chapter V.3. --- hTERT and hTEPl mRNA Expression in Oligodendroglial and Ependymal Tumors --- p.128 / Chapter V.4. --- Protein Expression ofpl6 and pRb in Oligodendroglial and Ependymal Tumors --- p.132 / Chapter V.5. --- Discussion on Telomerase Activity-Related Factors --- p.135 / Chapter V.6. --- Significance of Study and Clinical Application --- p.137 / Chapter V.7. --- Future Direction --- p.141 / Chapter VI. --- CONCLUSION --- p.143 / Chapter VII. --- REFERENCES --- p.146
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322878 |
| Date | January 1999 |
| Contributors | Chong, Yin Yue., Chinese University of Hong Kong Graduate School. Division of Anatomical and Cellular Pathology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xii, 161 leaves : ill. (some col., some mounted) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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