Chow Hoi Yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 127-153). / Abstracts in English and Chinese. / Abstract --- p.iii / Acknowledgments --- p.vi / Abbreviations --- p.vii / Table of contents --- p.viii / List of Figures --- p.xi / List of Tables --- p.xiv / Chapter Chapter One --- Literature Review / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- The life cycle of rice --- p.3 / Chapter 1.3 --- Physiological and molecular studies on rice leaf development --- p.6 / Chapter 1.3.1 --- Physiological study of rice leaf development --- p.6 / Chapter 1.3.1.1 --- Leaf primordium formation and SAM --- p.6 / Chapter 1.3.1.2 --- Leaf expansion and water status --- p.7 / Chapter 1.3.1.3 --- Leaf senescence and phytohormone --- p.8 / Chapter 1.3.1.4 --- Rice leaf and temperature --- p.9 / Chapter 1.3.2 --- Molecular study of rice leaf development / Chapter 1.3.2.1 --- Leaf primordium formation and SAM --- p.10 / Chapter 1.3.2.2 --- Leaf elongation and related genes --- p.13 / Chapter 1.3.2.3 --- Leaf senescence and related genes --- p.13 / Chapter 1.3.2.4 --- Photoreceptor genes --- p.14 / Chapter 1.3.2.5 --- Temperature-related genes --- p.17 / Chapter 1.4 --- Prospectus --- p.18 / Chapter Chapter Two --- Isolation of Genes Differentially Expressed During the Development of Rice by RAP-PCR / Chapter 2.1 --- Introduction --- p.20 / Chapter 2.2 --- Materials and Methods --- p.23 / Chapter 2.2.1 --- Strains and culture conditions --- p.23 / Chapter 2.2.2 --- Isolation of total RNAs --- p.23 / Chapter 2.2.3 --- cDNA Library construction --- p.24 / Chapter 2.2.3.1 --- First strand synthesis --- p.24 / Chapter 2.2.3.2 --- cDNA amplification by LD PCR --- p.25 / Chapter 2.2.3.3 --- Proteinase K digestion --- p.25 / Chapter 2.2.3.4 --- Sfi digestion --- p.26 / Chapter 2.2.3.5 --- cDNA size fractionation by CHROMA-SPIN´ёØ-400 --- p.26 / Chapter 2.2.3.6 --- Ligation of cDNA to λTripEx2vector --- p.26 / Chapter 2.2.3.7 --- Titering the unamplified library --- p.27 / Chapter 2.2.3.8 --- Determining the percentage of recombinant clones --- p.27 / Chapter 2.2.3.9 --- Library amplification --- p.28 / Chapter 2.2.3.10 --- Titering the amplified library --- p.28 / Chapter 2.2.3.11 --- Converting λ TripEx2 recombinant clones to pTripEx2 recombinant plasmids --- p.28 / Chapter 2.2.4 --- RNA fingerprinting by RAP-PCR --- p.29 / Chapter 2.2.5 --- Reverse dot-blot analysis --- p.30 / Chapter 2.2.5.1 --- Membrane preparation --- p.30 / Chapter 2.2.5.2 --- Probe preparation --- p.30 / Chapter 2.2.5.3 --- Hybridization --- p.31 / Chapter 2.2.5.4 --- Stringency washes and chemiluminescent detection --- p.31 / Chapter 2.2.6 --- Sequencing of differentially expressed genes --- p.32 / Chapter 2.2.6.1 --- Extraction of plasmid DNA --- p.32 / Chapter 2.2.6.2 --- DNA cycle sequencing --- p.33 / Chapter 2.3 --- Results --- p.34 / Chapter 2.3.1 --- Total RNA isolation --- p.34 / Chapter 2.3.2 --- cDNA library --- p.37 / Chapter 2.3.3 --- RNA fingerprinting --- p.40 / Chapter 2.3.4 --- Reverse dot-blot analysis --- p.45 / Chapter 2.3.5 --- Sequence analyses --- p.48 / Chapter 2.4 --- Discussion --- p.68 / Chapter Chapter Three --- cDNA Microarray Analysis and expression Pattern Analysis by Northern Blot Hybridization / Chapter 3.1 --- Introduction --- p.71 / Chapter 3.2 --- Materials and Methods --- p.76 / Chapter 3.2.1 --- RNA extraction by RNeasy® Mini Kit for cDNA microarray --- p.76 / Chapter 3.2.2 --- Microarray analysis --- p.76 / Chapter 3.2.2.1 --- Array preparation --- p.76 / Chapter 3.2.2.2 --- Probe preparation --- p.78 / Chapter 3.2.2.3 --- Hybridization --- p.79 / Chapter 3.2.2.4 --- Stringency washes and TSA Detection --- p.79 / Chapter 3.2.2.5 --- Microarray Analyses --- p.80 / Chapter 3.2.3 --- RNA extraction by Tri-reagent for Northern Blot hybridization --- p.81 / Chapter 3.2.4 --- RNA fragmentation by formaldehyde gel electrophoresis --- p.81 / Chapter 3.2.5 --- Northern blotting --- p.82 / Chapter 3.2.6 --- Preparation of probe --- p.83 / Chapter 3.2.7 --- Hybridization and stringency washes --- p.84 / Chapter 3.2.8 --- Stripping and reprobing of Northern Blot --- p.85 / Chapter 3.3 --- Results --- p.86 / Chapter 3.3.1 --- Total RNA isolation --- p.86 / Chapter 3.3.2 --- Microarray analysis --- p.86 / Chapter 3.3.3 --- Normalization of microarray data --- p.91 / Chapter 3.3.4 --- Detection of probe labeling efficiency --- p.92 / Chapter 3.3.5 --- Northern blot analysis --- p.100 / Chapter 3.3.5.1 --- Genes expressed highly in primordia stage --- p.100 / Chapter 3.3.5.2 --- Genes with low expression in primordia stage --- p.101 / Chapter 3.3.5.3 --- Genes with high expression in half expanded stage --- p.101 / Chapter 3.3.5.4 --- Genes steadily increased in expression throughout the development --- p.103 / Chapter 3.3.5.5 --- Genes with low expression in fully expanded stage --- p.104 / Chapter 3.4 --- Discussion --- p.114 / Chapter Chapter four --- General Discussion --- p.122 / References --- p.127
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324449 |
| Date | January 2003 |
| Contributors | Chow, Hoi Yee., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiv, 153 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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