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Analysis of flavan-3-ols by capillary electrophoresis.

Lee Wai Hang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 71-76). / Abstracts in English and Chinese. / Acknowledgement --- p.i / Abstract --- p.ii / Table of Contents --- p.v / Abbreviations --- p.viii / List of Figures --- p.ix / List of Tables --- p.xiv / Chapter / Chapter 1. --- Introduction --- p.1 / Chapter 1.1 --- The French Paradox --- p.1 / Chapter 1.2 --- Flavonoids --- p.2 / Chapter 1.3 --- Grape seed extract --- p.4 / Chapter 1.4 --- Instrunmental analysis --- p.7 / Chapter 1.4.1 --- High Performance Liquid Chromatography --- p.7 / Chapter 1.4.2 --- Colorimetry --- p.9 / Chapter 1.5 --- Capillary Electrophoresis --- p.10 / Chapter 1.5.1 --- Instrunmentation --- p.10 / Chapter 1.5.2 --- Electroosmotic Flow --- p.11 / Chapter 1.5.3 --- Electrophoretic mobility --- p.13 / Chapter 1.6 --- Objective of the study --- p.15 / Chapter 2. --- Experimental --- p.18 / Chapter 2.1 --- Reagents and material --- p.18 / Chapter 2.1.1 --- Reagents --- p.18 / Chapter 2.1.2 --- Instrunmentation --- p.18 / Chapter 2.1.3 --- Reference compounds --- p.19 / Chapter 2.1.4 --- Samples --- p.19 / Chapter 2.2 --- Selection of solvent for sample preparation --- p.20 / Chapter 2.3 --- Procedures --- p.21 / Chapter 2.3.1 --- Preparation of running buffer solution --- p.21 / Chapter 2.3.2 --- Preparation of standard solution --- p.21 / Chapter 2.3.3 --- Preparation of sample solution --- p.22 / Chapter 2.3.4 --- Flushing procedures --- p.22 / Chapter 3. --- Results and Discussion --- p.24 / Chapter 3.1 --- Preliminary experiments --- p.24 / Chapter 3.2 --- Effect of pH --- p.27 / Chapter 3.3 --- Addition of surfactant --- p.30 / Chapter 3.4 --- Effect of SDS concentration --- p.35 / Chapter 3.5 --- Addition of cyclodextrins --- p.39 / Chapter 3.6 --- Urea --- p.46 / Chapter 3.7 --- Addition of organic modifier --- p.47 / Chapter 3.8 --- Effect of borate concentration --- p.49 / Chapter 3.9 --- Effect of cyclodextrin concentration --- p.53 / Chapter 3.10 --- Optimized condition --- p.58 / Chapter 3.11 --- Reproducibility of the method --- p.58 / Chapter 3.12 --- Quantitative analysis of reference compounds --- p.60 / Chapter 3.13 --- Application of the CE method in grape seed products --- p.62 / Chapter 4. --- Conclusion --- p.69 / References --- p.71

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324610
Date January 2004
ContributorsLee, Wai Hang., Chinese University of Hong Kong Graduate School. Division of Chinese Medicine.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xv, 76 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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