Ho Wing Ki Vicky. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 191-211). / Abstracts in English and Chinese. / Abstracts --- p.i / Abstracts in Chinese --- p.iii / Acknowledgements --- p.v / Table of Abbreviations --- p.vi / Table of Contents --- p.ix / List of Figures and Tables --- p.xiv / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter 1.1. --- The Male Accessory Sex Glands --- p.2 / Chapter 1.1.1. --- The Prostatic Complex --- p.4 / Chapter 1.1.2. --- The Coagulating Gland --- p.4 / Chapter 1.1.3. --- The Seminal Vesicles --- p.5 / Chapter 1.1.4. --- The Ampullary Gland --- p.6 / Chapter 1.1.5. --- The Cowper's gland --- p.6 / Chapter 1.1.6. --- The Littre's Gland --- p.7 / Chapter 1.2. --- The Uterus --- p.7 / Chapter 1.2.1. --- The Structure of Uterus --- p.7 / Chapter 1.2.2. --- The Endometrium --- p.8 / Chapter 1.3. --- Identifications of Proteins by Two Dimensional Gel Electrophoresis and Matrix-assisted Laser Adsorption / ionization Time-of-flight (MALDI-TOF) Mass Spectrometry --- p.9 / Chapter 1.3.1. --- Principle of Two Dimensional Gel Electrophoresis --- p.9 / Chapter 1.3.2. --- Principle of MALDI-TOF Mass Spectrometry --- p.11 / Chapter 1.4. --- Hypothesis and Aim of this Study --- p.12 / Chapter 1.4.1. --- Hypothesis --- p.13 / Chapter 1.4.2. --- Aim --- p.13 / Legends and Figures --- p.14 / Chapter Chapter 2 --- The Effect of Omission of Male Accessory Sex Gland Secretions on Post Coital Uterine Fluid --- p.17 / Chapter 2.1. --- Introduction --- p.18 / Chapter 2.1.1. --- Uterine Fluid at the Time of Mating --- p.18 / Chapter 2.1.2. --- Ventral Prostate Secretory Proteins --- p.20 / Chapter 2.1.3. --- Proteins Specifically Bind to Sperm Membrane --- p.22 / Aim of Study --- p.23 / Chapter 2.2. --- Materials and Methods --- p.24 / Chapter 2.2.1. --- Animal Model --- p.24 / Chapter 2.2.1.1. --- Female golden hamsters --- p.24 / Chapter 2.2.1.2. --- Male golden hamsters --- p.25 / Chapter 2.2.2. --- Collections of Biological Samples --- p.26 / Chapter 2.2.2.1. --- Female Hamster Serum --- p.26 / Chapter 2.2.2.2. --- Uterine Fluid --- p.26 / Chapter 2.2.2.3. --- Uterine Tissues --- p.27 / Chapter 2.2.2.4. --- Ejaculated Sperm in Uterus --- p.27 / Chapter 2.2.2.5. --- ASG Secretions --- p.27 / Chapter 2.2.2.6. --- ASG Tissues --- p.28 / Chapter 2.2.2.7. --- Epididymal Sperm --- p.28 / Chapter 2.2.3. --- Isolation and Purification of Sperm Binding Ventral Prostate Proteins (SBVPP) --- p.28 / Chapter 2.2.3.1. --- Plasma Membrane Protein from Epididymal Sperm --- p.28 / Chapter 2.2.3.2. --- Affinity Chromatography --- p.29 / Chapter 2.2.3.3. --- FPLC Gel Filtration --- p.30 / Chapter 2.2.4. --- Histology of Flushed Uterus --- p.30 / Chapter 2.2.5. --- Quantification of Protein --- p.31 / Chapter 2.2.6. --- Two dimensional Electrophoresis --- p.31 / Chapter 2.2.6.1. --- First DimensiońؤIsoelectric Focusing --- p.32 / Chapter 2.2.6.2. --- Second DimensiońؤSDS PAGE --- p.32 / Chapter 2.2.6.3. --- Visualization of Protein Spots --- p.33 / Chapter 2.2.7. --- Identification of Proteins --- p.34 / Chapter 2.2.7.1. --- Protein Spots Detection --- p.34 / Chapter 2.2.7.2. --- MALDI-TOF --- p.34 / Chapter 2.2.7.2.1. --- In-Gel Digestion for MALDI-TOF --- p.34 / Chapter 2.2.7.2.2. --- MALDI-TOF Analysis --- p.35 / Chapter 2.2.7.2.3. --- MALDI-TOF Data Analysis --- p.36 / Chapter 2.2.8. --- Confirmation of Prostate Specific Antigen (PSA) --- p.37 / Chapter 2.2.9. --- Confirmation of Prostatic Acid Phosphatase (PAP) --- p.37 / Chapter 2.2.10. --- Confirmation of Tumor Necrosis Factor Stimulated Gene-6 (TSG-6) --- p.37 / Chapter 2.2.11. --- Scanning Electron Microscopy (SEM) --- p.38 / Chapter 2.3. --- Results --- p.39 / Chapter 2.3.1. --- Morphology of Flushed Uterus --- p.39 / Chapter 2.3.2. --- Protein Concentration --- p.39 / Chapter 2.3.3. --- "Serum, Pre and Post Coital Uterine Fluid Protein Profiles" --- p.39 / Chapter 2.3.4. --- ASG Secretory Proteins and Their Fate After mating --- p.40 / Chapter 2.3.4.1. --- ASG Secretory Proteins and 1 hp.c. Uterine Fluid --- p.40 / Chapter 2.3.4.2. --- VP Secretory Proteins and SBVPP --- p.40 / Chapter 2.3.4.3. --- PAP and PSA --- p.41 / Chapter 2.3.5. --- Protein Profile at 1 h p.c --- p.41 / Chapter 2.3.6. --- Protein Profile at 4 h p. c --- p.42 / Chapter 2.3.7. --- Identification of Proteins Differentially Expressed by the Three Groups at 1 h p .c --- p.43 / Chapter 2.3.7.1. --- Confirmation of TSG-6 --- p.43 / Chapter 2.3.8. --- SEM --- p.44 / Chapter 2.4. --- Discussion --- p.45 / Chapter 2.5. --- Conclusion --- p.55 / Legends and Figures --- p.56 / Chapter Chapter 3 --- The Effect of Omission of Male Accessory Sex Glands Secretions on Periimplanation Uterine Fluid Proteins --- p.114 / Chapter 3.1. --- Introduction --- p.115 / Chapter 3.1.1. --- Cytokine & Growth Factor --- p.115 / Chapter 3.1.1.1. --- Vascular Endothelial Growth Factor (VEGF) --- p.115 / Chapter 3.1.1.2. --- Leukaemia Inhibitory Factor (LIF) --- p.116 / Chapter 3.1.1.3. --- Insulin-like Growth Factors (IGFs) --- p.116 / Chapter 3.1.1.4. --- Interleukin 1 (IL-1) --- p.117 / Chapter 3.1.1.5. --- Adhesion Molecule --- p.118 / Chapter 3.1.2. --- Uterine Secretory Proteins --- p.118 / Chapter 3.1.2.1. --- Estrogen Dependent Protein --- p.119 / Chapter 3.1.2.2. --- Progestin-Dependent Endometrial Protein --- p.120 / Chapter 3.1.2.3. --- Uteroglobin --- p.121 / Chapter 3.1.2.4. --- Uteroferrin --- p.122 / Chapter 3.1.2.5. --- Prolactin --- p.123 / Chapter 3.1.2.6. --- Glycodelin --- p.123 / Chapter 3.2. --- Aim of Study --- p.124 / Chapter 3.3. --- Method and Materials --- p.124 / Chapter 3.3.1. --- Animal and Surgery --- p.125 / Chapter 3.3.2. --- Collection of Biological Samples --- p.125 / Chapter 3.3.2.1. --- Uterine Fluid --- p.125 / Chapter 3.3.2.2. --- Uterine Tissue --- p.125 / Chapter 3.3.3. --- Quantification of Protein --- p.125 / Chapter 3.3.4. --- Two Dimensional Electrophoresis --- p.125 / Chapter 3.3.5. --- Identification of Proteins --- p.126 / Chapter 3.3.6. --- Confirmation of Growth Differentiation Factor-8 (DGF-8) by PCR --- p.126 / Chapter 3.3.7. --- Confirmation of GDF-8 by Western Blotting --- p.126 / Chapter 3.4. --- Results --- p.128 / Chapter 3.4.1. --- Protein Concentration --- p.128 / Chapter 3.4.2. --- 60 Hours Post Coitum --- p.128 / Chapter 3.4.2.1. --- Protein Profile --- p.128 / Chapter 3.4.2.2. --- Differentially Expressed Protein Spots and Proteins Identification --- p.129 / Chapter 3.4.3. --- 72 Hours Post Coitum --- p.129 / Chapter 3.4.3.1. --- Protein Profile --- p.129 / Chapter 3.4.3.2. --- Differentially Expressed Protein Spots and Proteins Identification --- p.130 / Chapter 3.4.3.3. --- Confirmation of Growth differentiation factor 8 (GDF-8) --- p.130 / Chapter 3.5. --- Discussion --- p.132 / Chapter 3.6. --- Conclusion --- p.139 / Legends and Figures --- p.140 / Chapter Chapter 4 --- General Conclusion --- p.186 / Chapter 4.1. --- Conclusion --- p.187 / Chapter 4.2. --- Further Studies --- p.190 / References --- p.191 / Appendices --- p.212
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324632 |
| Date | January 2004 |
| Contributors | Ho, Wing Ki Vicky., Chinese University of Hong Kong Graduate School. Division of Anatomy. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xvi, 228 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
Page generated in 0.0018 seconds