by Lau Chun Hong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 179-195). / Abstracts in English and Chinese. / Abstract --- p.i / Abstract in Chinese 摘要 --- p.iv / Acknowledgements --- p.vi / Table of Contents --- p.vii / List of Abbreviations --- p.xiii / List of Figures --- p.xvi / List of Tables --- p.xviii / Publications --- p.xix / Chapter Chapter1 --- Introduction --- p.1 / Chapter 1.1 --- Epidemiology of Diabetes Mellitus --- p.1 / Chapter 1.2 --- Definition of Diabetes Mellitus --- p.2 / Chapter 1.3 --- Glucose Homeostasis and Diabetes Mellitus --- p.2 / Chapter 1.4 --- Classification of Diabetes Mellitus --- p.5 / Chapter 1.4.1 --- Type 1 Diabetes Mellitus --- p.5 / Chapter 1.4.2 --- Type 2 Diabetes Mellitus --- p.6 / Chapter 1.4.3 --- Other Specific Types --- p.7 / Chapter 1.4.4 --- Gestational Diabetes --- p.9 / Chapter 1.4.5 --- Clinical Stages of Diabetes --- p.9 / Chapter 1.5 --- Diagnostic Criteria of Diabetes Mellitus --- p.10 / Chapter 1.6 --- Complications of Diabetes Mellitus --- p.12 / Chapter 1.7 --- Pharmacological Treatment of Diabetes --- p.13 / Chapter 1.7.1 --- Treatment of Type 1 Diabetes --- p.13 / Chapter 1.7.2 --- Treatment of Type 2 Diabetes --- p.14 / Chapter 1.7.2.1 --- Sulphonylureas --- p.17 / Chapter 1.7.2.2 --- Biguanides --- p.18 / Chapter 1.7.2.3 --- α-Glucosidase Inhibitors --- p.19 / Chapter 1.7.2.4 --- Thiazolidinediones --- p.20 / Chapter 1.8 --- Diabetes and Traditional Chinese Medicine --- p.21 / Chapter 1.9 --- Project Objective --- p.27 / Chapter Chapter2 --- Botanical and Phytochemical Studies --- p.28 / Chapter 2.1 --- Introduction --- p.28 / Chapter 2.2 --- Materials --- p.31 / Chapter 2.3 --- Authentication of Herbal Material --- p.41 / Chapter 2.3.1 --- Materials --- p.41 / Chapter 2.3.2 --- Phytochemical Studies --- p.43 / Chapter 2.3.2.1 --- Sample Preparation --- p.43 / Chapter 2.3.2.2 --- Thin Layer Chromatography --- p.46 / Chapter 2.3.3 --- Results --- p.51 / Chapter 2.4 --- Extraction of Herbal Material --- p.56 / Chapter 2.4.1 --- Materials and Methods --- p.56 / Chapter 2.4.2 --- Results --- p.46 / Chapter 2.5 --- Quantification of Sugar Content in Herbal Extracts --- p.58 / Chapter 2.5.1 --- Introduction --- p.58 / Chapter 2.5.2 --- Materials and Methods --- p.58 / Chapter 2.5.3 --- Results --- p.61 / Chapter 2.6 --- Discussion --- p.65 / Chapter Chapter3 --- In vitro Studies on Formula 2 and its Individual Herbs --- p.68 / Chapter 3.1 --- Introduction --- p.68 / Chapter 3.2 --- Intestinal Glucose Absorption Studies --- p.69 / Chapter 3.2.1 --- Introduction --- p.69 / Chapter 3.2.2 --- Materials and Methods --- p.70 / Chapter 3.2.2.1 --- Preparation of BBMV --- p.71 / Chapter 3.2.2.2 --- BBMV Glucose Uptake Assay --- p.72 / Chapter 3.2.2.3 --- Bicinchoninic Acid (BCA) Protein Assay --- p.73 / Chapter 3.2.2.4 --- Preparation of Herbal Chloroform Extract --- p.74 / Chapter 3.2.2.5 --- Glucose Uptake Assay with Herbal Extracts --- p.75 / Chapter 3.2.3 --- Results --- p.76 / Chapter 3.3 --- Hepatic Gluconeogenesis Studies --- p.79 / Chapter 3.3.1 --- Introduction --- p.79 / Chapter 3.3.2 --- Materials and Methods --- p.82 / Chapter 3.3.2.1 --- Cell Culture --- p.83 / Chapter 3.3.2.2 --- Glucose Production Assay --- p.83 / Chapter 3.3.2.3 --- PEPCK Assay --- p.85 / Chapter 3.3.3 --- Results --- p.86 / Chapter 3.4 --- Cellular Glucose Uptake Studies --- p.88 / Chapter 3.4.1 --- Introduction --- p.88 / Chapter 3.4.2 --- Materials and Methods --- p.89 / Chapter 3.4.2.1 --- Cell Culture --- p.89 / Chapter 3.4.2.2 --- Differentiation of 3T3-L1 --- p.90 / Chapter 3.4.2.3 --- 2-Deoxy-D-glucose Uptake Assay --- p.91 / Chapter 3.4.3 --- Results --- p.92 / Chapter 3.5 --- Discussion --- p.96 / Chapter 3.5.1 --- Intestinal Glucose Absorption Studies by BBMV --- p.96 / Chapter 3.5.2 --- Hepatic Gluconeogenesis Studies by H4IIE Cells --- p.97 / Chapter 3.5.3 --- Cellular Glucose Uptake Studies by Hs68 and 3T3-L1 Cells --- p.99 / Chapter 3.5.4 --- Conclusions --- p.100 / Chapter Chapter4 --- In vivo Studies on Selected Herbs --- p.103 / Chapter 4.1 --- Introduction --- p.103 / Chapter 4.1.1 --- Animal Models of Type 2 Diabetes --- p.103 / Chapter 4.1.2 --- Chemically-induced Diabetic Models --- p.104 / Chapter 4.1.3 --- Neonatal-STZ Diabetic Rats --- p.107 / Chapter 4.2 --- Basal Glycaemia Test --- p.109 / Chapter 4.2.1 --- Animals --- p.109 / Chapter 4.2.2 --- Testing Method --- p.110 / Chapter 4.2.3 --- Results --- p.112 / Chapter 4.3 --- Oral Glucose Tolerance Test --- p.114 / Chapter 4.3.1 --- Animals --- p.114 / Chapter 4.3.2 --- Testing Method --- p.114 / Chapter 4.3.3 --- Results --- p.116 / Chapter 4.4 --- Discussion --- p.119 / Chapter Chapter5 --- Bioassay-guided Fractionation of Cortex Moutan --- p.125 / Chapter 5.1 --- Introduction --- p.125 / Chapter 5.1.1 --- Phytochemical Studies of Cortex Moutan --- p.125 / Chapter 5.2 --- Organic Extraction of Cortex Moutan --- p.128 / Chapter 5.2.1 --- Extraction Method --- p.128 / Chapter 5.2.2 --- Results --- p.129 / Chapter 5.3 --- BBMV Glucose Uptake Assay with Fraction CM C --- p.131 / Chapter 5.3.1 --- Materials and Methods --- p.131 / Chapter 5.3.2 --- Results --- p.131 / Chapter 5.4 --- In vivo Studies of Fraction CM-C --- p.133 / Chapter 5.4.1 --- Materials and Methods --- p.133 / Chapter 5.4.2 --- Results --- p.133 / Chapter 5.5 --- Fractionation of Fraction CM-C --- p.137 / Chapter 5.5.1 --- Materials and Methods --- p.137 / Chapter 5.5.2 --- Results --- p.139 / Chapter 5.6 --- BBMV Glucose Uptake Assay with CM-C Sub-fractions --- p.142 / Chapter 5.6.1 --- Results --- p.142 / Chapter 5.7 --- Isolation of Active Compound in Fraction CM-C4 --- p.144 / Chapter 5.7.1 --- Materials and Methods --- p.145 / Chapter 5.7.2 --- Results --- p.146 / Chapter 5.8 --- Structure Elucidation of CM-C4a --- p.148 / Chapter 5.8.1 --- Materials and Methods --- p.148 / Chapter 5.8.2 --- Results --- p.149 / Chapter 5.9 --- Effect of Paeonol in Oral Glucose Tolerance Test --- p.152 / Chapter 5.9.1 --- Materials and Methods --- p.152 / Chapter 5.9.2 --- Results --- p.153 / Chapter 5.10 --- Discussion --- p.155 / Chapter Chapter6 --- General Discussion --- p.163 / Chapter 6.1 --- Introduction --- p.163 / Chapter 6.2 --- Summary of Research Findings --- p.164 / Chapter 6.3 --- Limitations and Improvements --- p.167 / Chapter 6.4 --- Future Directions --- p.169 / Chapter 6.5 --- Conclusion --- p.170 / Appendices --- p.172 / Appendix 1 Low Resolution EI Mass Spectrum of Paeonol Reference --- p.173 / Appendix 2 Low Resolution EI Mass Spectrum of CM-C4a --- p.174 / Appendix 3 High Resolution EI Mass Spectrum of Paeonol Reference --- p.175 / Appendix 4 High Resolution EI Mass Spectrum of CM-C4a --- p.176 / Appendix 5 1H-NMR Spectrum of Paeonol Reference --- p.177 / Appendix 6 1H-NMR Spectrum of CM-C4a --- p.178 / References --- p.179
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324951 |
| Date | January 2004 |
| Contributors | Lau, Chun Hong., Chinese University of Hong Kong Graduate School. Division of Chinese Medicine. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xix, 195 leaves : ill. (some col.) ; 30 cm. |
| Coverage | China |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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