Chau, Hiu Yan Anita. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 109-122). / Abstracts in English and Chinese. / Chapter Chapter 1. --- Introduction --- p.1 / Chapter 1.1 --- Introduction on mushroom life cycle --- p.1 / Chapter 1.2 --- Introduction of mushroom sclerotium --- p.2 / Chapter 1.3 --- Different extraction methods of mushroom polysaccharides --- p.3 / Chapter 1.4 --- Bioactivities of mushroom polysaccharides and factors affecting their biological activities --- p.4 / Chapter 1.4.1 --- Molecular weight --- p.4 / Chapter 1.4.2 --- Linkages --- p.5 / Chapter 1.4.3 --- Branching rate --- p.5 / Chapter 1.4.4 --- Conformation --- p.6 / Chapter 1.5 --- Mechanisms for antitumor activites of mushrooms polysaccharides.… --- p.7 / Chapter 1.5.1 --- Cancer-preventing activity --- p.7 / Chapter 1.5.2 --- Immuno-enhancing activity (BRM) --- p.8 / Chapter 1.5.3 --- Direct tumor inhibition activity --- p.8 / Chapter 1.6 --- Cell cycle regulation and induction of apoptosis --- p.9 / Chapter 1.6.1 --- The cell cycle machinery --- p.9 / Chapter 1.6.2 --- Cell cycle arrest and regulation --- p.11 / Chapter 1.6.3 --- Apoptosis and regulation --- p.13 / Chapter 1.7 --- Literature review on Pleurotus tuber-regium --- p.16 / Chapter 1.7.1 --- Introduction of Pleurotus tuber-regium --- p.16 / Chapter 1.7.2 --- Antitumor effect of mushroom polysaccharides isolated from different developmental stages of Pleurotus tuber-regium --- p.17 / Chapter 1.7.2.1 --- Sclerotium --- p.17 / Chapter 1.7.2.2 --- Mycelium --- p.19 / Chapter 1.7.2.3 --- Culture medium --- p.19 / Chapter 1.7.2.4 --- Fruiting body --- p.20 / Chapter 1.8 --- Literature review on Size reduction process --- p.21 / Chapter 1.8.1 --- Introduction of micron technology --- p.21 / Chapter 1.8.1.1 --- Ball milling --- p.21 / Chapter 1.8.1.2 --- Jet milling --- p.22 / Chapter 1.8.1.3 --- High-pressure micronizing --- p.22 / Chapter 1.8.1.4 --- Oscillatory milling --- p.23 / Chapter 1.8.2 --- Effect of particle sizes on physicochemical properties and biological activities of plant materials --- p.23 / Chapter 1.8.2.1 --- Physicochemical properties --- p.24 / Chapter 1.8.2.2 --- Biochemical activities --- p.24 / Chapter 1.9 --- Objectives --- p.26 / Chapter Chapter 2. --- Materials and methods --- p.28 / Chapter 2.1 --- Materials --- p.28 / Chapter 2.1.1 --- Mushroom sclerotia --- p.28 / Chapter 2.1.2 --- Micronisation --- p.29 / Chapter 2.1.3 --- Cell lines --- p.31 / Chapter 2.1.4 --- Antibodies --- p.33 / Chapter 2.1.5 --- Animal model --- p.33 / Chapter 2.2 --- Methods --- p.34 / Chapter 2.2.1 --- Micronisation --- p.34 / Chapter 2.2.2 --- Hot water extraction for mushroom sclerotia --- p.35 / Chapter 2.2.3 --- Measurement of monosaccharide profile --- p.36 / Chapter 2.2.3.1 --- Acid deploymerisation --- p.36 / Chapter 2.2.3.2 --- Neutral sugar derivatization --- p.36 / Chapter 2.2.3.3 --- Gas chromatography (GC) --- p.37 / Chapter 2.2.4 --- Total sugar content by Phenol-sulphuric acid Method --- p.38 / Chapter 2.2.5 --- Acidic sugar content by measuring uronic acid content --- p.39 / Chapter 2.2.6 --- Protein content by Lowry-Folin Method --- p.40 / Chapter 2.2.7 --- Size exclusion chromatography by high pressure liquid chromatograhy (HPLC) --- p.41 / Chapter 2.2.8 --- In vitro antitumor assay --- p.41 / Chapter 2.2.8.1 --- Trypan blue exclusion assay --- p.42 / Chapter 2.2.8.2 --- MTT Assay --- p.42 / Chapter 2.2.9 --- Cell cycle analysis by Flow Cytometry --- p.43 / Chapter 2.2.10 --- Protein expression involved in apoptosis --- p.45 / Chapter 2.2.10.1 --- Cell lysates preparation --- p.45 / Chapter 2.2.10.2 --- Determination of protein concentrations --- p.46 / Chapter 2.2.10.3 --- Western blot --- p.46 / Chapter 2.2.11 --- In vivo antitumor assay --- p.50 / Chapter 2.2.11.1 --- BALB/c mice --- p.50 / Chapter 2.2.11.2 --- Athymic nude mice --- p.50 / Chapter 2.2.12 --- Statistical methods --- p.51 / Chapter Chapter 3 --- Results and Discussion --- p.52 / Chapter 3.1 --- Yield of hot water-soluble extracts from Pleurotus tuber-regium --- p.52 / Chapter 3.2 --- Chemical composition of hot water-soluble extracts from PTR --- p.56 / Chapter 3.2.1 --- Total carbohydrate content --- p.56 / Chapter 3.2.2 --- Uronic acid content --- p.57 / Chapter 3.2.3 --- Protein content --- p.58 / Chapter 3.3 --- Monosaccharide profiles of hot water-soluble extracts from PTR by gas chromatography (GC) --- p.61 / Chapter 3.4 --- Molecular weight profile of hot water-soluble extracts from PTR by size exclusion chromatography (SEC) --- p.64 / Chapter 3.5 --- Antitumor effects of mushroom sclerotial polysaccharides --- p.72 / Chapter 3.5.1 --- In vitro antiproliferation study --- p.72 / Chapter 3.5.1.1 --- In vitro antiproliferation study by HL-60 --- p.72 / Chapter 3.5.1.2 --- In vitro antiproliferation study by THP-1 --- p.75 / Chapter 3.5.1.3 --- In vitro antiproliferation study by MCF-7 --- p.77 / Chapter 3.5.1.4 --- In vitro antiproliferation study by K562 --- p.77 / Chapter 3.5.1.5 --- In vitro antiproliferation study by SI80 --- p.79 / Chapter 3.5.1.6 --- In vitro antiproliferation study by normal cells --- p.79 / Chapter 3.5.1.7 --- Dose-response relationship between hot water-soluble extract from PTR and tumor cell inhibition --- p.80 / Chapter 3.5.2 --- In vivo antitumor study --- p.83 / Chapter 3.5.2.1 --- BALB/c mice --- p.83 / Chapter 3.5.2.2 --- Athymic nude mice --- p.84 / Chapter 3.6 --- Flow cytometric analysis of tumor cells treated by various hot wter-soluble extracts from PTR --- p.88 / Chapter 3.6.1 --- Antiproliferative effect of various hot water-soluble extracts from 10PTR on HL-60 --- p.88 / Chapter 3.6.2 --- Antiproliferative effect of various hot water-soluble extracts from 10PTR on THP-1 --- p.93 / Chapter 3.7 --- Effects of various hot water-soluble extracts from 10PTR on expression of Bcl-2 and Bax proteins in HL-60 cells --- p.99 / Chapter 3.8 --- "Correlation between particle size, structure and antitumor activity of mushroom sclerotial extracts" --- p.101 / Chapter Chapter 4. --- Conclusions and Future Works --- p.105 / List of References --- p.109 / Related Publications --- p.123
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326385 |
| Date | January 2008 |
| Contributors | Chau, Hiu Yan Anita., Chinese University of Hong Kong Graduate School. Division of Food and Nutritional Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xviii, 123 leaves : ill. (chiefly col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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