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Study on the in vitro anti-tumor effect of Acanthopanax senticosus.

Wan, Chung Tin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 126-139). / Abstracts in English and Chinese. / Thesis / Assessment Committee --- p.i / Acknowledgement --- p.ii / Abstract (English) --- p.iii / Abstract (Chinese) --- p.v / List of Abbreviations --- p.vii / List of Tables --- p.ix / List of Figures --- p.x / Chapter Chanter 1 --- Introduction / Chapter 1.1. --- Overview of cancer --- p.1 / Chapter 1.1.1 --- Breast cancer --- p.1 / Chapter 1.1.2 --- Hepatocellular carcinoma (HCC) --- p.2 / Chapter 1.2. --- Role of natural products in the fight against cancer --- p.3 / Chapter 1.2.1 --- Introduction of Astragalus membranaceus --- p.4 / Chapter 1.2.2 --- Introduction of Acanthopanax senticosus --- p.9 / Chapter 1.2.3 --- Introduction of Grifola Frondosa --- p.13 / Chapter 1.3. --- Apoptosis and cancer --- p.17 / Chapter 1.3.1 --- Caspases --- p.20 / Chapter 1.3.2 --- Intrinsic apoptotic pathway --- p.21 / Chapter 1.3.3 --- Extrinsic apoptotic pathway --- p.23 / Chapter 1.3.4 --- Execution of apoptosis --- p.26 / Chapter 1.4 --- Cell cycle and cancer --- p.27 / Chapter 1.5 --- Objective of this project: --- p.29 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Materials: --- p.30 / Chapter 2.1.1 --- Culture medium --- p.30 / Chapter 2.1.2 --- Buffers and reagents --- p.31 / Chapter 2.1.3 --- Herbs --- p.34 / Chapter 2.2 --- Methods --- p.35 / Chapter 2.2.1 --- Cell cultures --- p.35 / Chapter 2.2.1.1 --- Cell lines --- p.35 / Chapter 2.2.1.2 --- Cell culture techniques --- p.36 / Chapter 2.2.1.3 --- Cryopreservation of cell lines --- p.36 / Chapter 2.2.2 --- Proliferation assay --- p.37 / Chapter 2.2.2.1 --- MTT assay --- p.37 / Chapter 2.2.2.2 --- Isolation of PBMC --- p.38 / Chapter 2.2.2.3 --- XTT --- p.39 / Chapter 2.2.3 --- DNA fragmentation --- p.39 / Chapter 2.2.4 --- Flow cytometry --- p.41 / Chapter 2.2.4.1 --- Detection of mitochondrial membrane depolarization by the use of JC-1 --- p.41 / Chapter 2.2.4.2 --- Annexin-V-FITC PI labeling of apoptotic cells --- p.42 / Chapter 2.2.4.3 --- Cell cycle analysis --- p.43 / Chapter 2.2.5 --- Western blotting --- p.43 / Chapter 2.2.5.1 --- Total protein extraction --- p.44 / Chapter 2.2.5.2 --- Determining protein concentration --- p.44 / Chapter 2.2.5.3 --- SDS-PAGE --- p.45 / Chapter 2.2.5.4 --- Electroblotting --- p.45 / Chapter 2.2.5.5 --- Probing --- p.46 / Chapter 2.2.5.6 --- ECL --- p.46 / Chapter 2.2.6 --- Preparation of herbal extracts --- p.47 / Chapter 2.2.6.1 --- Preparation of extract of Astragalus membranaceus and Grifola frondosa --- p.47 / Chapter 2.2.6.2 --- Preparation of Acanthopanax senticosus aqueous extract --- p.47 / Chapter 2.2.6.3 --- Partition of Acanthopanax --- p.47 / Chapter 2.2.6.4 --- Column purification of ethyl-acetate fraction --- p.48 / Chapter 2.2.6.5 --- Analytical thin layer chromatography --- p.49 / Chapter 2.2.7 --- Statistical Analysis --- p.50 / Chapter Chanter 3 --- Results / Chapter 3.1 --- Extractions --- p.51 / Chapter 3.2 --- Anti-proliferative effect of herbal extracts on cancer cell lines --- p.51 / Chapter 3.3 --- Partition of Acanthopanax methanol extract --- p.56 / Chapter 3.4 --- Anti-proliferative effect of Acanthopanax partition fractions on breast cancer cells --- p.59 / Chapter 3.5 --- Column chromatography of ethyl acetate fraction --- p.59 / Chapter 3.6 --- Anti-proliferative effect of various sub-fractions on breast cancer cells --- p.64 / Chapter 3.7 --- Effect of sub-fractions on PBMC proliferation --- p.76 / Chapter 3.8 --- Kinetic study of anti-proliferative effect of Fc --- p.76 / Chapter 3.9 --- Flow cytometric analysis --- p.79 / Chapter 3.91 --- JC-1 staining --- p.79 / Chapter 3.92 --- Annexin-P1 labeling --- p.79 / Chapter 3.93 --- Cell cycle analysis --- p.80 / Chapter 3.10 --- DNA fragmentation assay --- p.88 / Chapter 3.11 --- Western blotting --- p.91 / Chapter Chanter 4 --- Discussion --- p.99 / Chapter Chapter 5 --- Conclusion --- p.124 / References --- p.126

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326547
Date January 2008
ContributorsWan, Chung Tin., Chinese University of Hong Kong Graduate School. Division of Biochemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiv, 139 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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