Wang, Juan. / "July 2010." / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 58-66). / Abstracts in English and Chinese. / Thesis/ Assessment Committee --- p.ii / Statement --- p.iii / Abstract --- p.iv / 摘要 --- p.vi / Acknowledgements --- p.vii / Table of Contents --- p.ix / List of Tables --- p.xii / List of Figures --- p.xiii / List of Abbreviations --- p.xv / Chapter CHAPTER1 --- General Introduction --- p.1 / Chapter 1.1 --- The Plant Secretory Pathway --- p.1 / Chapter 1.2 --- Exocytosis --- p.4 / Chapter 1.3 --- Exocyst complex --- p.5 / Chapter 1.3.1 --- Exocyst complex in plants --- p.6 / Chapter 1.4 --- Project Objectives --- p.6 / Chapter CHAPTER 2 --- Materials and Methods --- p.8 / Chapter 2.1 --- Plasmid construction --- p.8 / Chapter 2.2 --- Expression and Purification of Recombinant Protein --- p.11 / Chapter 2.3 --- Transient Expression of Arabidopsis Protoplasts --- p.13 / Chapter 2.4 --- Transformation of Tobacco BY-2 and Arabidopsis Cells --- p.14 / Chapter 2.5 --- Antibodies --- p.16 / Chapter 2.6 --- Immunofluorescent Studies --- p.19 / Chapter 2.7 --- FM4-64 Uptake Study --- p.20 / Chapter 2.8 --- Drug Treatments --- p.21 / Chapter 2.9 --- Electron Microscope of Resin-Embedded Cells --- p.21 / Chapter 2.10 --- Sucrose Starvation --- p.23 / Chapter 2.11 --- Chemical Screening --- p.23 / Chapter CHAPTER 3 --- Results --- p.25 / Chapter 3.1 --- Expression of (X)FP-tagged AtExo70E2 in Arabidopsis protoplasts --- p.25 / Chapter 3.2 --- AtExo70E2 labels the PM and organelles which do not lie on the secretory or endocytic pathways --- p.27 / Chapter 3.3 --- "Transgenic Arabidopsis and tobacco BY-2 cell lines expressing AtExo70E2, and AtExo70E2-specific antibodies help in identifying Exocyst-positive organelles (EXPO)" --- p.31 / Chapter 3.4 --- EXPO do not label with FM4-64 --- p.36 / Chapter 3.5 --- EXPO are exocytic double membraned organelles --- p.39 / Chapter 3.6 --- EXPO are true organelles in the cytoplasm that are not induced by sucrose starvation --- p.44 / Chapter 3.7 --- EXPO can be specifically affected by one chemical compound --- p.48 / Chapter CHAPTER 4 --- Discussion and Future Perspectives --- p.51 / Chapter 4.1 --- Discussion --- p.51 / Chapter 4.1.1 --- Exocyst and EXPO --- p.51 / Chapter 4.1.2 --- Are EXPO related to Autophagosomes? --- p.54 / Chapter 4.1.3 --- EXPO and exosomes: agents of unconventional secretion --- p.55 / Chapter 4.2 --- Future perspectives --- p.56 / References --- p.58
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327123 |
| Date | January 2010 |
| Contributors | Wang, Juan., Chinese University of Hong Kong Graduate School. Division of Life Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xvi, 66 leaves : ill. (chiefly col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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