Li, Yuefang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 46-48). / Abstracts in English and Chinese. / Abstract --- p.i / 摘要 --- p.ii / Acknowledgement --- p.iii / Table of contents --- p.iv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Introduction to protein crystallization --- p.1 / Chapter 1.1.1 --- Principles of protein crystallization --- p.2 / Chapter 1.1.2 --- Classical methods to crystallize protein --- p.4 / Chapter 1.2 --- Crystal growth in unique environments: the pursuit of better crystals --- p.6 / Chapter 1.2.1 --- Protein crystallization in space --- p.6 / Chapter 1.2.2 --- Crystallization in gel and capillary --- p.7 / Chapter 1.3 --- Microfluidic methods for protein crystallization: high through-put screenings --- p.9 / Chapter 1.3.1 --- Valve-controlled methods --- p.10 / Chapter 1.3.2 --- Droplet-based methods --- p.11 / Chapter 1.3.3 --- Microwell-based methods --- p.11 / Chapter 1.4 --- Objective of the project --- p.13 / Chapter Chapter 2 --- Rehydratable hydrogel in nanoliter microwells --- p.15 / Chapter 2.1 --- Introduction --- p.15 / Chapter 2.2 --- Experimental --- p.17 / Chapter 2.2.1 --- Fabrication of SU-8 mould --- p.17 / Chapter 2.2.2 --- Fabrication of the PDMS device --- p.19 / Chapter 2.2.3 --- Liquid dispensing in PDMS device --- p.20 / Chapter 2.2.4 --- Polymerization of PA gel --- p.21 / Chapter 2.2.5 --- Drying and Rehydration of PA gel --- p.22 / Chapter 2.3 --- Results and discussions --- p.23 / Chapter 2.3.1 --- Preparation of PA gel in PDMS device --- p.23 / Chapter 2.3.2 --- Immobilization of PA gel in microwells --- p.25 / Chapter 2.3.3 --- Dehydration and Rehydration of PA gel --- p.25 / Chapter 2.3.4 --- Liquid dispensing in the gel-preloaded microwells --- p.29 / Chapter 2.4 --- Conclusion --- p.31 / Chapter Chapter 3 --- Protein crystallization by gel-based FID --- p.32 / Chapter 3.1 --- Introduction --- p.32 / Chapter 3.2 --- Experimental --- p.34 / Chapter 3.2.1 --- Conditions used for crystallize proteins --- p.34 / Chapter 3.2.2 --- Protein crystallization by microbatch method --- p.34 / Chapter 3.2.3 --- Protein crystallization in microchip --- p.35 / Chapter 3.3 --- Results and discussions --- p.35 / Chapter 3.3.1 --- Crystallization in microplate --- p.36 / Chapter 3.3.2 --- Crystallization in microwells --- p.38 / Chapter 3.4 --- Conclusion --- p.41 / Chapter Chapter 4 --- Conclusions --- p.43 / Chapter 4.1 --- Summary of the work --- p.43 / Chapter 4.2 --- Future perspectives --- p.44 / Reference --- p.46
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327247 |
| Date | January 2010 |
| Contributors | Li, Yuefang., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, v, 48 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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