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Effect of 3-methylthymine on solution structures and thermodynamic stabilities of double-helical deoxyribonucleic acids.

Zhong, Yangliu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 50-57). / Abstracts in English and Chinese. / Title Page --- p.i / Thesis Committee --- p.ii / Abstract (English Version) --- p.iv / Abstract (Chinese Version) --- p.V / Acknowledgement --- p.vi / Table of Contents --- p.viii / List of Tables --- p.X / List of Figures --- p.xii / List of Abbreviations and Symbols --- p.xiii / Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- DNA methylation --- p.1 / Chapter 1.2 --- Repair of m3T --- p.2 / Chapter 1.3 --- Objectives of this work --- p.3 / Chapter 1.4 --- DNA structure --- p.3 / Chapter 1.4.1 --- Nomenclature scheme for DNA --- p.3 / Chapter 1.4.2 --- Base pair scheme --- p.4 / Chapter 1.4.3 --- Sugar conformation --- p.5 / Chapter 1.4.4 --- Backbone conformation --- p.7 / Chapter 2 --- Materials and Methods --- p.9 / Chapter 2.1 --- Sample design --- p.9 / Chapter 2.2 --- Sample preparation --- p.10 / Chapter 2.3 --- NMR analysis --- p.10 / Chapter 2.3.1 --- Resonance assignment --- p.12 / Chapter 2.3.2 --- Determination of sugar conformation --- p.13 / Chapter 2.3.3 --- Determination of backbone conformation --- p.14 / Chapter 2.4 --- UV melting study --- p.15 / Chapter 3 --- Effect of m3T on Double-Helical Structures and Stabilities --- p.17 / Chapter 3.1 --- Resonance assignments --- p.17 / Chapter 3.2 --- Effect of m3T on double-helical DNA structures --- p.19 / Chapter 3.2.1 --- Base pairing mode --- p.19 / Chapter 3.2.2 --- Sugar conformation --- p.21 / Chapter 3.2.3 --- Backbone conformation --- p.22 / Chapter 3.3 --- Effect of m3T on double-helical DNA stabilities --- p.25 / Chapter 3.4 --- Discussion --- p.26 / Chapter 3.4.1 --- Single-strand requirement in FTO repair --- p.26 / Chapter 3.4.2 --- Relationship between m3T pairing structure and stability --- p.27 / Chapter 4 --- Effect of m3T Mispair on Double-Helical DNA Structures and Stabilities --- p.28 / Chapter 4.1 --- Resonance assignments --- p.28 / Chapter 4.2 --- Effect of m3T mispair on double-helical DNA structures --- p.32 / Chapter 4.2.1 --- Pairing mode of T m3T --- p.34 / Chapter 4.2.2 --- Pairing mode of G m3T --- p.35 / Chapter 4.2.3 --- Pairing mode of C.m3T --- p.35 / Chapter 4.3 --- Effect of m3T mispair on double-helical DNA stabilities --- p.36 / Chapter 4.4 --- Discussion --- p.36 / Chapter 4.4.1 --- Predominant mutation --- p.37 / Chapter 4.4.2 --- Relationship between m3T pairing structure and stabilities --- p.37 / Chapter 5 --- Conclusion and Future Work --- p.39 / Chapter Appendix I --- Proton chemical shift values (ppm) of AmT --- p.40 / Chapter Appendix II --- Proton chemical shift values (ppm) of RefAT --- p.41 / Chapter Appendix III --- Proton chemical shift values of NmT samples --- p.42 / Chapter Appendix IV --- "Σ1' and %S of TmT, GmT and CmT" --- p.45 / Chapter Appendix V --- "1H-31P HSQC spectra of (a) TmT, (b) GmT and (c) CmT" --- p.46 / Chapter Appendix VI --- "1H-31P COSY spectra of (a) TmT, (b) GmT and (c) CmT" --- p.47 / Chapter Appendix VII --- "31P chemical shifts, 3JH3'P and %Bi of TmT, GmT and CmT" --- p.48 / Chapter Appendix VIII --- "UV melting curves of RefAT, AmT, TmT, GmT and CmT" --- p.49 / References --- p.50

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327506
Date January 2011
ContributorsZhong, Yangliu., Chinese University of Hong Kong Graduate School. Division of Chemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiv, 58 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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