鹽鹼化是世界上最主要非生物脅迫之一，它主要是由於土壤中的鹽（氯化鈉）過量積累所導致的, 不僅影響植物的生長而且也影響農作物的產量。大豆是世界上最重要的經濟類豆科植物之一，由於其種子內含有豐富的營養物質例如蛋白質，油，糖分和纖維，所以它為我們提供了極為重要和大量的油脂和蛋白。在鹽脅迫下大豆的產量會有明顯的降低。由於以上這些情況，我們希望搞清楚大豆對於鹽脅迫的反應機制。首先是通過蛋白質組學弄清楚鹽脅迫的生理過程和大豆如何耐受鹽脅迫的。一般來說，蛋白質組學包括了鑑定蛋白類的化合物和測量在生物系統中的蛋白含量的學科。近期，質譜的發展提供了一個去研究細胞內蛋白質的動態變化十分有用的平台。定量蛋白質組學的發展對於我們系統性的了解蛋白質的功能的分子是十分重要的並且預期會提供給我們各種生物過程和系統的分子機制的見解.其次，表觀遺傳性特別是組蛋白修飾。因為組蛋白修飾通過重新編排染色體的結構和組成參與了許多重要的生物過程並且這些翻譯後修飾對於植物的耐受鹽脅迫也發揮著十分重要的作用。因此我們希望了解組蛋白修飾是如何參與這一過程的。 / Salinity stress, which is caused by the accumulation of excessive amount of salts in the soil, is one of the most severe abiotic stresses that constraint not only crop plant growth but also crop productivity. Soybean (Glycine max) is one of the most important economical legume crops in the world because of its richness of nutritional compositions including protein, oil, sugar and fiber in the seed. Soybean yield of sensitive cultivars is decreased dramatically under salt stress. Because of this, we tried to figure out the mechanism of how soybean response to salinity stress. Firstly, Proteomics--to elucidate the affected physiological process in the salinity stress and the way to tolerant the stress. In general, proteomics involves the identification of protein components and the measurement of protein abundance in biological systems. Recent mass spectrometry (MS)-based technology developments have provided useful platforms for the study of quantitative changes in protein components within the cell. Quantitative proteomics is important for the system-based understanding of the molecular function of each protein component and expected to provide insights into molecular mechanisms of various biological processes and systems. Secondly, Epigenetics--particularly histone modifications. Because histone modifications play important roles in many fundamental biological processes by rearranging the structure and composition of chromatin and PTMs have more roles in response salinity stress. So we want to understand how PTMs involve in this process from epigenetics. / Detailed summary in vernacular field only. / Peng, Xu. / "October 2012." / Thesis (M.Phil.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 44-54). / Abstracts also in Chinese. / Thesis committee --- p.i / Declaration --- p.ii / Abstract (in English) --- p.iii / Abstract (in Chinese) --- p.iv / Acknowledgements --- p.v / Table of contents --- p.vi / General abbreviations --- p.vii / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Soybean --- p.1 / Chapter 1.2 --- Salinity stress and plants’ response to salinity stress --- p.1 / Chapter 1.3 --- Mass Spectrometry base Proteomics --- p.3 / Chapter 1.3.1 --- Introduction to proteomics --- p.3 / Chapter 1.3.2 --- Proteomic studies in plants --- p.5 / Chapter 1.4 --- Introduction to Epigenetics --- p.6 / Chapter 1.5 --- Present studies --- p.8 / Chapter Chapter 2 --- Proteomic studies in soybean --- p.9 / Chapter 2.1 --- Materials and methods --- p.9 / Chapter 2.1.1 --- Plant materials and stress treatment --- p.9 / Chapter 2.1.2 --- Protein extraction and nuclei extraction and histone isolation --- p.10 / Chapter 2.1.3 --- Protein Preparation for Mass Spectrometry --- p.11 / Chapter 2.1.4 --- Analysis of Protein using nanoLC-MS/MS and Data analysis --- p.12 / Chapter 2.2 --- Results and discussions --- p.13 / Chapter 2.2.1 --- One thousand two hundred seventeen proteins were identified by LC MS/MS-based proteomics technique --- p.13 / Chapter 2.2.2 --- Functional analyses of identified proteins --- p.22 / Chapter 2.2.3 --- One hundred sixty-three proteins are changed under salinity stress as identified by LC MS/MS-based proteomics technique --- p.26 / Chapter 2.2.4 --- Important stress relate proteins were identified by LC MS/MS-based proteomics technique --- p.30 / Chapter 2.2.5 --- Histone PTMs in soybean under salinity stress --- p.34 / Chapter Chapter 3 --- Conclusions and perspectives --- p.35
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_328680 |
| Date | January 2013 |
| Contributors | Peng, Xu., Chinese University of Hong Kong Graduate School. Division of Life Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | electronic resource, electronic resource, remote, 1 online resource (viii, 206 leaves) : ill. (some col.) |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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