A mouse in vitro co-culture model between endometrial epithelial cells (EEC) and peripheral blood lymphocytes and monocytes (PBLM) immune cells was established, and Ct lipopolysaccharide (LPS) was added to the cells to mimic Ct bacterial infection and to stimulate an immune response. This model enabled us to study the cross-talk between EEC and PBLM and the physiological changes that occur in the endometrium upon Ct LPS stimulation. Results showed that EEC-PBLM co-culture and Ct LPS stimulation caused changes in transepithelial resistance (TER) as well as the expression and function of the cystic fibrosis transmembrane conductance regulator (CFTR) ion channel of epithelial cells. CFTR gene transcription was up-regulated at early hours after Ct LPS stimulation, while its channel activity was down-regulated at later hours. These results suggested the possible involvement of CFTR acting as a receptor for the internalization of Ct, which may ultimately lead to the disappearance of CFTR on the apical membrane. The EEC-PBLM co-culture showed that cross-talk was important for host defense in the endometrium. Direct cross-talk by cell-cell contact between EEC and PBLM was vital for immune cell survival as well as strengthening epithelials' barrier function. (Abstract shortened by UMI.) / Chlamydia trachomatis (Ct) infection is one of the most prevalent causes for sexually transmitted disease (STD) in the female reproductive tract. Ct is unique in that it is a bacterium, but infects and replicates like a virus inside a host cell. Ct infection can lead to a variety of reproductive diseases, such as pelvic inflammatory diseases (PID), tubule scarring, salpingitis, endometriosis, ectopic pregnancy and infertility. Effective immune defense in the uterus is necessary to eliminate these bacteria and to ensure optimal uterine environment for sperm motility, fertilization, and embryo implantation to occur. The immune system of the endometrium responds to Ct infection by the recruitment of many types of leukocytes, such as T-lymphocytes, B-lymphocytes, monocytes, macrophages and neutrophils, to the site of infection. Cross-talk between endometrial epithelial cells and immune cells may alter the activities of epithelial cells causing changes in channels function and anion secretion. / Ho Alice. / "August 2005." / Adviser: Chan Hsiao Chang. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 3532. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (p. 155-167). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_343703 |
| Date | January 2005 |
| Contributors | Ho, Alice., Chinese University of Hong Kong Graduate School. Division of Physiology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, theses |
| Format | electronic resource, microform, microfiche, 1 online resource (167 p. : ill.) |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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