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A Study on the Potential Role of Stress Granules and Processing Bodies in Eliminating Oxidatively Damaged RNA

Oxidative stress (OS) is strongly implicated in age-related neurodegeneration and
other diseases. Under OS, the production of excessive oxidants leads to increased
damages to cellular components. Recently, RNA has been discovered as a major target of
oxidative damage, including the creation of abasic sites. In this work, we developed a
method for quantifying abasic RNA in cell. Using this method, we have examined the
potential role of the RNA-processing cellular foci, stress granule (SG) and processing
bodies (PB) in eliminating abasic RNA in situ. We demonstrated that RNA is a major
target of oxidative damage, constituting the majority of OS-induced abasic nucleic acids
in HeLa cell. Importantly, the level of abasic RNA is strongly correlated with SG
abundance. Furthermore, inhibition of SG/PB formation causes accumulation of abasic
RNA, suggesting that SG/PB participates in removing oxidized RNA and protects cells
under OS, which offers novel targets for therapeutic intervention in age-related diseases. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2016. / FAU Electronic Theses and Dissertations Collection

Identiferoai:union.ndltd.org:fau.edu/oai:fau.digital.flvc.org:fau_33710
ContributorsPourkalbassi, Delaram (author), Li, Zhongwei (Thesis advisor), Florida Atlantic University (Degree grantor), Charles E. Schmidt College of Medicine, Department of Biomedical Science
PublisherFlorida Atlantic University
Source SetsFlorida Atlantic University
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation, Text
Format94 p., application/pdf
RightsCopyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder., http://rightsstatements.org/vocab/InC/1.0/

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