Capillary electrophoresis is currently a very powerful technique for the analysis of seized drugs. A rapid analytical CE method for the screening and quantification of GHB and GBL was achieved using 300mM CTAC/25mM phosphate buffer pH 6.3. Reversed phase HPLC was achieved using 25mM phosphate buffer pH 6.5 and a Cl8 Aqua column. Chiral separation of 9 amphetamine type stimulants was obtained using a highly sulfated gamma-cyclodextrin as a chiral selector. MECC and CZE were compared for the analysis of psilocybin, while a rapid and robust method is presented for the analysis of major opium alkaloids, using dynamically coated capillary columns. The column is coated with a polycation followed by a polyanion coating, using a commercial reagent kit. Using a background electrolyte pH of 2.5 with the addition of hydroxypropyl-beta- cyclodextrin and dimethyl-beta-cyclodextrin, the analysis of morphine, papaverine, codeine, noscapine, and thebaine in opium samples was obtained with great resolution. Finally, separation of common benzodiazedpines was also investigated using CZE and a pH 2.5 phosphate buffer.
| Identifer | oai:union.ndltd.org:fiu.edu/oai:digitalcommons.fiu.edu:etd-5329 |
| Date | 28 March 2005 |
| Creators | Garcia, Agnes D. |
| Publisher | FIU Digital Commons |
| Source Sets | Florida International University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | FIU Electronic Theses and Dissertations |
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