Changes in zinc homeostasis are strongly associated with abnormal brain function and a variety of neurological and neuropsychiatric disorders, including depression. It is hypothesized that the neurogenic potential of chronic antidepressant administration contributes to its therapeutic effects in depression. Thus, the goal of this work was to determine the extent to which zinc is needed for antidepressant drug induction of neural stem cell proliferation and differentiation. Human NTERA-2/D1 (NT2) cell culture, an established in vitro model system to study neuronal development, was utilized. Zinc deficiency impaired NT2 cell proliferation measured by the number of Ki67-positive cells. Treatment with fluoxetine or lithium did not result in a significant increase in cell proliferation rate. However, six-day treatment with these antidepressants had a stimulatory effect on NT2 cell differentiation revealed by immunofluorecent detection of the neuron-specific marker TuJ1. Furthermore, zinc deficient cultures treated with fluoxetine or lithium appeared to have a decreased expression of this neuronal marker. Taken together, these results suggest that the essential trace element zinc is needed for neuronal stem cell proliferation and differentiation. / A Thesis submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements
for the degree of Master of Science. / Spring Semester, 2009. / July 2, 2008. / Antidepressants, Depression, Neurogenesis / Includes bibliographical references. / Cathy W. Levenson, Professor Directing Thesis; Jasminka Ilich-Ernst, Committee Member; Myra Hurt, Outside Committee Member.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_254031 |
| Contributors | Mullin, Tatyana (authoraut), Levenson, Cathy W. (professor directing thesis), Ilich-Ernst, Jasminka (committee member), Hurt, Myra (outside committee member), Department of Nutrition, Food, and Exercise Science (degree granting department), Florida State University (degree granting institution) |
| Publisher | Florida State University, Florida State University |
| Source Sets | Florida State University |
| Language | English, English |
| Detected Language | English |
| Type | Text, text |
| Format | 1 online resource, computer, application/pdf |
| Rights | This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). The copyright in theses and dissertations completed at Florida State University is held by the students who author them. |
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