This study investigated the distribution and composition of glycoconjugates axonally transported to subcellular fractions of goldfish optic tectum, specifically the SPM, SV and soluble fractions. In addition this study also assessed the effectiveness of using different solvents for the extraction of intact axonally transported glycoconjugates. / The subcellular distribution of radioactivity in the goldfish optic tectum 12 hrs and 24 hrs postintraocular injection of ('35)S-sulfate or ('3)H-fucose, respectively, indicated that the RSA of both SV and SPM was greater than those of other membranous fractions. A small portion of transported label was associated with the soluble fraction. At 36 hrs PIO injection of ('35)SO(,4), the RSA of the SV and SPM increased by 18% and 9% respectively, whereas the RSA of the soluble fraction decreased by 16%. / Compositional analysis of the SPM glycoconjugates demonstrated that fucosylated molecules had a broad MW distribution mainly below 100k daltons, whereas sulfated molecules had MWs > 100k daltons. In contrast to SPM, transported sulfated and fucosylated GC of the SV fraction demonstrated an MW distribution primarily below 100k daltons. / Anion exchange chromatography resulted in the separation of 3 populations of transported soluble GC. Those molecules eluting with increasing ionic strength from the anion exchange column showed a progressive increase in MW as seen by gel filtration. The late eluting peak of highly acidic molecules contained in addition to sulfated glycopeptides all of the soluble glycosaminoglycans indicating the presence of transport labeled proteoglycans. / To determine conditions which would lead to solubilization of membrane-affiliated AT GC, pooled optic tecta were sequentially homogenized in different solvents 12 hrs PIO injection of ('35)SO(,4). Approximately 20% of the recovered transported label was soluble in buffered sucrose, 12% was extracted with sucrose containing 1.0 M NaCl, 34% was extracted with 4 M guanidine and 11% remained unextracted. The sucrose and salt extracted mainly GP while guanidine extracts and unextracted residue were enriched in PGs. / Source: Dissertation Abstracts International, Volume: 44-09, Section: B, page: 2672. / Thesis (Ph.D.)--The Florida State University, 1983.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_75185 |
| Contributors | RIPELLINO, JAMES A., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 226 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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