This dissertation examines the effectiveness of several data processing treatments for quantitative analysis of mixtures in three areas of spectroscopy: fluorescence, absorption, and Raman. The data treatments considered are of two types, those that quantitate the analyte signal in the presence of a background signal and those that are used to quantitate all the constituents in the mixture. / The fluorescence study compares the results of several data treatments which utilize spectral shapes to distinguish analyte and nonanalyte signals. Two models are used for the comparison of data treatments. The first uses two components which are spectrally overlapped and the bandwidth for each of the two bands is markedly different. The second uses two components which are overlapped and the bandwidth for each band is similar. / The absorption section describes two experiments. The first experiment tests the effectiveness of several data treatments in extracting the signal of a minor constituent in the presence of a major constituent in a commercially available cold medicine. The second experiment compares the effectiveness of the data treatments on a mixture composed to two compounds with almost identical spectra. Results show that data treatments using all the spectral information are more effective in quantitating the components with similar overlapped spectra. The determination of the minor constituent was unsatisfactory with the equipment available. / The Raman spectroscopy section describes the quantitation of the active ingredients in a pharmaceutical preparation. The results of the data treatments on both solution and solid mixtures allow a comparison of the advantages and disadvantages for each data treatment and sample type. Raman spectroscopy was successful in quantitating the analyte signal in solid mixtures. / Source: Dissertation Abstracts International, Volume: 45-09, Section: B, page: 2896. / Thesis (Ph.D.)--The Florida State University, 1984.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_75419 |
| Contributors | KING, THOMAS HAROLD., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 180 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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