Genes coding for replication-dependent, partially replication-dependent, and replication-independent histones have been isolated from mouse. These genes were isolated from libraries of mouse DNA in lambda bacteriophage. Three previously-isolated mouse histone genes, several heterologous histone genes, and synthetic oligonucleotides were used to identify the clones. The four replication-dependent and two partially replication-dependent genes have been sequenced. Transcripts of these genes from mouse myeloma cells, from C127 mouse fibroblasts, and from differentiating murine erythroleukemia cells have been characterized using the S1 nuclease assay. Transcripts of several of the replication-independent genes from differentiating murine erythroleukemis cells have been studied, and one of these genes has been sequenced. There are no intervening sequences in the coding region of this gene, but there may be intervening sequences in the 5' flanking region. Primer extension studies have been used to determine the length of the transcript from the genes for replication-independent histones. The replication-dependent and partially replication-dependent histone genes are clustered, and the clusters contain members of only one expression class. Only genes coding for the core histones have been found in these clusters. The replication-independent H3 genes appear to be isolated from other histone genes. / Source: Dissertation Abstracts International, Volume: 47-01, Section: B, page: 0083. / Thesis (Ph.D.)--The Florida State University, 1986.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_75764 |
| Contributors | WELLMAN, SUSAN ELIZABETH., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 146 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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