Microinjection of mutant templates into sea urchin one-cell zygotes showed that maximal expression of the early U1 snRNA gene in vivo was dependent upon two cis-acting DNA elements. The distal sequence element (DSE) was located to a position $-$300 nt from the start site of transcription, while the proximal sequence element (PSE) was localized to a region $-$50 to $-$60 relative to the transcription start site. Deletion of these regions completely abolished expression of the gene in blastula stage embryos. Additionally, these elements were also shown to bind nuclear factors. / The process of U1 snRNA 3$\sp\prime$ end formation was also examined and was shown to occur by a novel mechanism. Proper U1 RNA 3$\sp\prime$ end formation occurs independently of the conserved purine-rich 3$\sp\prime$ signal located downstream of the gene. Additionally, proper 3$\sp\prime$ end formation of U1 transcripts occurs when transcription is driven by a histone promoter. The transcripts that bypass the U1 3$\sp\prime$ end were shown to be unstable. Moreover, transcripts that initiate from the U1 promoter are capable of forming histone 3$\sp\prime$ ends. / The PSE elements of the early U1 and U2 genes were shown to be functionally distinct. Microinjection of PSE swap clones in combination with competitive gel shift assays indicates that these elements interact with different nuclear factors. / Source: Dissertation Abstracts International, Volume: 52-11, Section: B, page: 5681. / Major Professor: William F. Marzluff, Jr. / Thesis (Ph.D.)--The Florida State University, 1991.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_76554 |
| Contributors | Wendelburg, Brian James., Florida State University |
| Source Sets | Florida State University |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | 179 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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