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Isolation and Purification of Planctomycetes associated with Harbor and Lagoon Seagrasses of the Red Sea

Planctomycetes
are
members
of
a
unique
superphylum
along
with
Verrucomicrobia
and
Chlamydia,
situated
in
the
domain
Bacteria.
They
have
distinct
structural
and
morphological
features,
and
discoveries
made
through
phylogenetic
analysis
indicate
their
important
role
in
nutrient
cycling.
Their
known
relationships
with
marine
photosynthetic
organisms
led
to
the
formation
of
this
study's
hypothesis,
namely,
that
Planctomycetes
can
be
isolated
from
the
biofilm
of
seagrass
species
of
the
Red
Sea
using
cultivation
techniques
adapted
for
these
organisms.
Preparation
of
solid
and
liquid
media
using
M13
with
both
agar
and
gellan,
and
2216
Difco
Marine
Broth
full-­
strength,
1/10-­strength,
and
with
antibiotics,
resulted
in
the
successful
isolation
of
Planctomycetes
as
confirmed
by
morphological
examination
and
transmission
electron
microscopy.
The
work
performed
in
this
study
provides
a
solid
foundation
for
further
studies
to
elucidate
the
metabolic
pathways
and
ecological
significance
of
Planctomycetes.

Identiferoai:union.ndltd.org:kaust.edu.sa/oai:repository.kaust.edu.sa:10754/237292
Date07 1900
CreatorsBream, Holly
ContributorsBajic, Vladimir B., Lafi, Feras, Saikaly, Pascal, Stingl, Ulrich
Source SetsKing Abdullah University of Science and Technology
LanguageEnglish
Detected LanguageEnglish
TypeThesis

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