Life in oceans originated about 3.5 billion years ago where microbes were the
only life form for two thirds of the planet’s existence. Apart from being
abundant and diverse, marine microbes are involved in nearly all
biogeochemical processes and are vital to sustain all life forms. With the
overgrowing number of data arising from culture-independent studies, it
became necessary to improve culturing techniques in order to obtain pure
cultures of the environmentally significant bacteria to back up the findings and
test hypotheses. Particularly in the ultra-oligotrophic Red Sea, the ubiquitous
SAR11 bacteria has been reported to account for more than half of the
surface bacterioplankton community. It is therefore highly likely that SAR11,
and other microbial life that exists have developed special adaptations that
enabled them to thrive successfully. Advances in conventional culturing have
made it possible for abundant, unculturable marine bacteria to be grown in the
lab. In this study, we analyzed the effectiveness of the media LNHM and
AMS1 in isolating marine bacteria from the Red Sea, particularly members of
the SAR11 clade. SAR11 strains obtained from this study AMS1, and
belonged to subgroup 1a and phylotype 1a.3. We also obtained other
interesting strains which should be followed up with in the future. In the long
run, results from this study will enhance our knowledge of the pelagic
ecosystem and allow the impacts of rising temperatures on marine life to be
understood.
| Identifer | oai:union.ndltd.org:kaust.edu.sa/oai:repository.kaust.edu.sa:10754/306936 |
| Date | 12 1900 |
| Creators | Mohamed, Roslinda B. |
| Contributors | Stingl, Ulrich, Biological and Environmental Sciences and Engineering (BESE) Division, Ngugi, David, Saikaly, Pascal |
| Source Sets | King Abdullah University of Science and Technology |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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