<P> Mitochondria contain DNA which is distinct from
nuclear DNA. The capacity of isolated rat liver mitochondria
to synthesize RNA and the types of RNA synthesized were
examined in order to determine the genetic function of
mitochondrial DNA. </p> <p> It was demonstrated that isolated rat liver
mitochondria synthesize RNA, incorporating [3H]UTP, [3H] ATP,[3H]CTP and [3H]GTP in a DNA-dependent reaction. In addition,
the DNA-independent incorporation of [3H]CTP and [3H]ATP
suggested metabolic turnover of the CCA end of mitochondrial
tRNA. </p> <P> Analysis of the , newly-synthesized RNA by sucrose
density gradient centrifugation and agarose-polyacrylamide
gel electrophoresis demonstrated that mitochondrial ribosomal
RNA was synthesized in a DNA-dependent process. It is
concluded that one of the genetic functions of mitochondrial
DNA is to code for mitochondrial ribosomal RNA. </p> / Thesis / Doctor of Philosophy (PhD)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/19440 |
| Date | 02 1900 |
| Creators | Fukamachi, Seijiro |
| Contributors | Freeman, Karl B., Biochemistry and Biomedical Sciences |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
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