Nucleic acid amplification is responsible for pushing the limit-of-detection of molecular
diagnostic assays to unprecedented levels. We developed an assay based on protein-responsive programmable dynamic DNA assembly (PRPDA) to detect proteins via an
intermediate process involving nucleic acids for taking advantage of nucleic acid amplification strategies. PRPDA has previously been designed for sensitive protein analysis in
fluorescent assay formats. To further push the detection limit and to achieve assay
miniaturization and multiplexing, we sought to combine PRPDA with electrochemical
readout. We were able to achieve LOD of 1 pM by employing wrinkled gold electrode
for the PRDA protein detection scheme. Which is 2800 times improvement compare to
the 2.8 nM demonstrated by fluorescent transduction. / Thesis / Master of Applied Science (MASc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/22890 |
| Date | January 2018 |
| Creators | Hasan, Md Roqibul |
| Contributors | Soleymani, Dr. Leyla, Biomedical Engineering |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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