In many systems, in vitro or in vivo, it has become important to experimentally obtain
dynamical information at many different positions simultaneously. This is a challenge
as conventionally, dynamic information in biological systems is probed with a confocal
microscope to perform either fluorescence correlation spectroscopy (FCS) or fluorescence
recovery after photobleaching (FRAP), which can be damaging due to phototoxicity, and
yields information at a single position. Advances in camera sensors have allowed their
use in place of single point detectors and the implement of imaging FCS by way of single
plane illumination microscopy (SPIM). In this modality, a light sheet with a thickness of
only a few microns illuminates the sample and the fluorescence is projected orthogonally
onto the camera chip. By imaging small regions of interest at a very high frame rate, we
can determine dynamic parameters such as diffusion coefficients and local concentrations
in a 2D array of pixels. In this thesis, I discuss the theoretical background, hardware
setup, design and characterization of a SPIM which I have built in order to perform
imaging FCS. / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/26128 |
| Date | January 2020 |
| Creators | Mahmood, M. Ahmad |
| Contributors | Fradin, Cecile, Physics and Astronomy |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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