<p>Environment-responsive paper-supported composite membranes, consisting of interpenetrating network of poly(<em>N</em>-vinyl caprolactam) and cellulose fibres, suitable for hydrophobic interaction membrane chromatography of proteins were prepared. These membranes could be made to switch between hydrophobic and hydrophilic states in a reversible manner by addition or removal of anti-chaotropic salts. A series of systematic studies were conducted to examine the effects of polymer loading and salt concentration on hydraulic and antibody binding properties of these membranes. A membrane possessing a satisfactory balance of hydraulic permeability and antibody binding was further examined for use in an immunoglobulin G (IgG) purification process. The membrane demonstrated low fouling tendency, satisfactory IgG binding (c.a. 12 mg/ml bed volume), and was successfully used for IgG purification from simulated cell culture supernatant. The results obtained demonstrate that the poly(<em>N</em>-vinyl caprolactam)-paper composite and indeed similar material could be used as inexpensive membranes adsorbers for protein purification.</p> / Master of Applied Science (MASc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/9110 |
| Date | January 2009 |
| Creators | Mah, Kor-Zheng |
| Contributors | Ghosh, R., Chemical Engineering |
| Source Sets | McMaster University |
| Detected Language | English |
| Type | thesis |
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