The use of the neutralisation assay in the serotyping of unknown enterovirus isolates has been successfully applied for years since
its description by Lim and Benyesh-Melnick1. However, the procedure is labour-intensive and time consuming. In addition, the continuous
depletion of the Benyesh-Melnick sera makes it prohibitive for most diagnostic laboratories to continue using the assay.
Owing to these factors, a majority of laboratories only type for the polioviruses and report any other enterovirus isolate as “a nonpolio
enterovirus”. With this approach, however, important findings such as the isolation of a new enterovirus or association of a
known enterovirus with a new clinical syndrome will remain unidentified.
In this study, a multiple-serum-pools approach similar to that described by Lim and Benyesh-Melnick1 (LBM) for the neutralisation
test, was applied in an immunofluorescence (IMF) test for the rapid serotyping of enteroviruses. This test was able to type any
unknown enterovirus isolate belonging to the different enterovirus serotypes tested in this study, in about three hours as compared to
the eight days currently taken by the LBM neutralisation test1.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:tut/oai:encore.tut.ac.za:d1000941 |
| Date | 01 December 2004 |
| Creators | Basetse, HR, Lecatsas, G, Odendaal, JSJ |
| Publisher | Journal of Medical Technology SA |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | |
| Rights | Medical Technology SA |
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