Abstract
Using thin-layer chromatography, 16 bacterial isolates from root nodules of 8 different Psoralea species were quantitatively
assessed for their exudation of the metabolites lumichrome, riboflavin and IAA in response to pH, salinity and temperature.
Our data showed that the bacterial strains tested differed in their levels of secretion of the three metabolites. For example,
strain AS2 produced significantly greater amounts of lumichrome at both pH 5.1 and 8.1, while strains RTl and PI
produced more lumichrome per cell at only pH 8.1. Strains API and RP2 also produced more riboflavin at pH 5.1 than at pH
8.1; conversely strain RTl secreted more riboflavin at pH 8.1 than at pH 5.1. Two P. repens strains (RPI and RP2) isolated
from very saline environments close to the Indian Ocean produced significant levels of lumichrome and riboflavin at both
low and high salinity treatments. However, strains ACI and Ll (from P. aculeata and P. laxa) even produced greater
amounts of lumichrome and riboflavin at higher salinity (i.e. 34.2 mM NaCl) and probably originated from naturally saline
soils. In this study, high acidity and high temperature induced the synthesis and release of high levels of IAA by bacterial
cells. In contrast, there was greater strain secretion of lumichrome at lower temperature (10°C) than at high temperature
(30°C). The variations in the secretion of lumichrome, riboflavin and IAA by bacterial strains exposed to different pH,
salinity and temperature regimes suggest that genes encoding these metabolites are regulated differently by the imposed
environmental factors. The data from this study also suggest that natural changes of pH, salinity and/or temperature in plant
rhizospheres could potentially elevate the concentrations of lumichrome, riboflavin and IAA in soils. An accumulation of
these molecules in the rhizosphere would have consequences for ecosystem functioning as both lumichrome and riboflavin
have been reported to act as developmental signals that affect species in all three plant, animal, and microbial kingdoms.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:tut/oai:encore.tut.ac.za:d1001481 |
| Date | January 2009 |
| Creators | Kanu, S, Dakora FD |
| Publisher | Symbiosis |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | |
| Rights | ©2009 Balaban, PhiiadelphialRehovot |
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