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Evaluation of anti-flammatory, antibacterial and cytotoxic activities of cordia africana leaf and stem bark extracts

Abstract
Cordia africana (Boraginaceae) is a tree used in traditional medicine to treat inflammation related
conditions and infectious diseases. This study was undertaken with the objectives of establishing the
scavenging effect of extracts and fractions of Codia africana on the mediator of inflammation
Lipoxygenases (LOX), and some non-biological free radicals such as 2,2-diphenyl-1-picrylhydrazyl
(DPPH), the [2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS) radicals and the Ferric ion
reducing antioxidant power (FRAP). Antimicrobial activities, total phenolics/flavonoids and
cytotoxicity of extracts of Codia africana were also evaluated. Extracts were obtained by maceration.
Anti-inflammatory activity was determined using a LOX-inhibitor screening assay kit according to the
manufacturer's instructions. A broth serial micro dilution method was used to determine the minimum
inhibitory concentration (MIC) against, Gram-positive and Gram-negative bacteria and Mycobacterium
species. The antioxidant activity was determined using free-radical-scavenging assays, and the 3-(4,5-
dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide reduction assay was used for cytotoxicity. Both
the extracts of C. africana inhibited LOX enzyme. The most active being the methanol extract of the
bark with IC50 value of 55 ± 0.9 μg/ml. Both the extracts of C. africana, had excellent to weak
antimicrobial activites (MICs ranging from 32 to 1024 μg/ml) against bacteria. All the extracts had
significant (P< 0.05) free-radical scavenging activity (IC50 ranging from 6.79 ± 0.07 to 331.98 ± 0.07
μg/ml). There was a positive correlation between the antioxidant activity and the total flavonoid and
total phenolic contents of Cordia africana. The cytotoxicity on Vero cells was low with LC50 of 81.79 ±
13.31 and 99.67 ± 16.10 μg/ml. The results support the use of C. africana leaves in traditional
medicine to treat inflammation related conditions and infectious diseases.

Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:tut/oai:encore.tut.ac.za:d1001973
Date01 June 2016
CreatorsImam, ID, Alhaji, SMI, Ahmad, A, Paul, DJ, Adeniyi, AS, Idris, M, Fulatan, SU, Alexandra, DP
PublisherBayero Journal of Pure and Applied Sciences
Source SetsSouth African National ETD Portal
LanguageEnglish
Detected LanguageEnglish
TypeText
FormatPdf
RightsBayero Journal of Pure and Applied Sciences

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