Includes bibliographical references (leaves 98-103). / The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics. For this purpose, the methylotrophic yeast Pichia pastoris has emerged as an additional microbial host for recombinant insulin production. A genetically modified Pichia pastoris MutS strain, engineered to produce the insulin precursor, was used as the experimental system in this study in order to optimise the insulin production process. The experimental system developed in this study employed a two-stage fed-batch feeding strategy in which growth was optimised by feeding glycerol to boost biomass followed by induction of the gene encoding insulin precursor by feeding methanol.
Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uct/oai:localhost:11427/10120 |
Date | January 2011 |
Creators | Bhardwaj, Vinayak |
Contributors | Harrison, STL, Minnaar, Sanet |
Publisher | University of Cape Town, Faculty of Engineering and the Built Environment, Centre for Bioprocess Engineering Research |
Source Sets | South African National ETD Portal |
Language | English |
Detected Language | English |
Type | Master Thesis, Masters, MSc |
Format | application/pdf |
Page generated in 0.0027 seconds