M.Sc. (Zoology) / The construction of the Pongolapoort Dam in 1974 has altered conditions downstream of the Phongolo River in terms of hydrology, geomorphology, water quality, and ecosystem services. Activities along the Phongolo River permit anthropogenic compounds such as pesticides used in disease control to enter the aquatic environment. This alteration of natural environmental conditions creates a need for a monitoring programme. This study aims to assess the use of Hydrocynus vittatus as an indicator organism of pollution in the Nyamithi Pan and Phongolo River. Water and sediment samples were analysed for a variety of metal elements. The physical characteristics of water samples were determined using a WTW Multi 340i multimeter. Nutrients in water were analysed using a Merck Spectroquant™ Pharo 100 Spectrophotometer. Sediment analysis was based on the standard protocols of the United States Environmental Protection Agency (2001). Physiological stress responses in Hydrocynus vittatus were determined using acetylcholine esterase, catalase, cellular energy allocation, 7-ethoxyresorufin-O-deethylase, malondialdehyde, metallothionein, superoxide dismutase, and protein carbonyls. Employing standard techniques H. vittatus was analysed for metals and organics using ICP-MS, ICP-GS and ICP-OES. Results for biological responses and bioaccumulation show significant differences between sites related to the environmental concentrations of elements. Organisms in the Phongolo River have elevated concentrations of DDT and the metabolites indicating recent exposure. Organisms from the Phongolo River are seen to be responding based on up or down regulated concentrations of biological markers. In particular, concentrations of CAT, MDA and PC are elevated in the Phongolo population with decreased AChE responses indicating toxicant exposure. Data accumulated in this study will contribute to the establishment of baseline chemical, physical and biological knowledge of the effects of contamination in South African waters.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uj/uj:11674 |
| Date | 15 July 2014 |
| Creators | Tate, Russell Brian |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Thesis |
| Rights | University of Johannesburg |
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