D.Phil. / The determination of several physiologically important molecules has been carried out using an oxygen electrode and various oxidase or oxygenase enzymes. The enzymatic reactions are monitored either by measuring the initial rates of oxygen uptake from the medium or by calculating the total amount of oxygen consumed in the reactions. Because of the use of enzymes, the determinations are rapid and sensitive and each one is highly specific. Versatility of analysis can be introduced into the system by suitable choice of the enzyme. Furthermore, the methods are simple, and colour or turbidity does not interfere, thus enabling even whole blood to be used directly as sample material ...
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uj/uj:13977 |
| Date | 01 September 2015 |
| Source Sets | South African National ETD Portal |
| Detected Language | English |
| Type | Thesis |
| Rights | University of Johannesburg |
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