Magister Scientiae - MSc / An amperometric biosensor was prepared by in situ deposition of cytochrome P4502D6 (CYP2D6, P4502D6) enzyme on a polyaniline (PANI)-doped glassy carbon electrode. The PANI film was electrochemically deposited on the electrode at 100 mV s-1 against Ag/AgCl. Cyclic voltammetric characterisation of the PANI film in 1 M HCl solution showed two distinct redox peaks, which prove
that the PANI film was electroactive and exhibited fast reversible electrochemistry. The surface concentration and film thickness of the adsorbed electroactive species was estimated to be 1.85 x 10 -7 mol cm -2 and approximately
16 nm, respectively. Cytochrome P4502D6 was electrostatically immobilised onto the surface of the
PANI film and cyclic voltammetry was used to monitor the serotonin reuptake inhibitors (fluoxetine, fluvoxamine and citalopram) in phosphate buffer solution. Fluoxetine was found to be a substrate of CYP2D6 at low concentrations but
inhibits enzyme activity at high concentrations; this was consistent with uncompetitive substrate inhibition kinetics. Thus PANI-mediated electrochemistry can be used to observe monooxygenation reaction of CYP2D6. / South Africa
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:uwc/oai:etd.uwc.ac.za:11394/1589 |
| Date | January 2005 |
| Creators | Mathebe, Ntlatseng Gretta Rhoda |
| Contributors | Iwuoha, Emmanuel, Dept. of Chemistry, Faculty of Science |
| Publisher | University of the Western Cape |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | University of the Western Cape |
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