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Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase

Magister Scientiae - MSc / In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate. / South Africa
Date January 2009
CreatorsMketsu, Moses Clive Masisange
ContributorsCowan, Don A., Tuffin, Marla, van Zyl, Lonnie, Dept. of Biotechnology, Faculty of Science
PublisherUniversity of the Western Cape
Source SetsSouth African National ETD Portal
Detected LanguageEnglish
RightsUniversity of the Western Cape

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