M.Sc. (Med.), Faculty of Health Sciences, University of the Witwatersrand, 2011 / Pneumocystis pneumonia (PCP) is one of the most prevalent diseases in HIV-positive and
other immunosuppressed patients. It is caused by the opportunistic fungal pathogen
Pneumocystis jirovecii. Dihydropteroate synthase (DHPS) mutations in P. jirovecii have
been linked to resistance to trimethoprim-sulphamethoxazole (cotrimoxazole), the main
treatment and prophylaxis used for PCP. DHPS mutations have been identified globally,
predominantly in developed countries. This study investigated the P. jirovecii DHPS
genotypes in PCP-positive patients from Chris Hani Baragwanath Hospital in Gauteng
Province, South Africa.
During the period March 2005 through June 2009, 266 patients were enrolled in the study
and 306 specimens were collected. P. jirovecii was identified in 67% (205/306) of these
specimens with quantitative real-time PCR (qPCR). The qPCR had a sensitivity of 98%
and a specificity of 70%, compared with the immunofluorescence assay (IFA). Using
sequencing and cloning techniques, 64% (110/173) of the nested PCR-positive specimens
contained P. jirovecii with mutant DHPS genotypes. There was no association between
patients harbouring P. jirovecii with mutant DHPS genotypes and in-hospital patient
outcome (p-value = 0.19). As part of this project the Roche MagNA Pure Compact
(RMPC) instrument and technology was validated for use as a new DNA extraction
method. The RMPC was quick and easy to use compared to the Qiagen manual extraction
method.
The specificity of the qPCR was compromised by the high number of apparent false
positive results obtained by the assay. However, as the IFA is an imperfect gold standard,
these are probably true cases of infection or colonisation. This study found a higher
proportion of P. jirovecii with DHPS mutant genotypes than wild type in PCP patients,
which is unusual. The most probable reason for this is the widespread use of sulfa drugs,
which are thought to select for these mutations. While this study did not find an
association between DHPS mutations and adverse patient outcome, there have been
contradictory findings. If further investigations reveal that DHPS mutations affect patient
treatment or outcome, it will have major implications for the management of PCP in the
country.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:wits/oai:wiredspace.wits.ac.za:10539/11555 |
| Date | 20 June 2012 |
| Creators | Poonsamy, Bhavani |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | application/pdf, application/pdf |
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