Humic acids have high ability to form stable complexes with copper ions, which influences their toxicity in environment. Fluorescent properties of sodium salt of humic acid, humate, isolated from South Moravian lignite, and its complexation behaviour with copper ions were investigated using emission, excitation and synchronous fluorescence spectroscopy. Both fluorescence emission and synchronous fluorescence spectra showed that humate form complex with copper since fluorescence intensity was quenched upon addition of copper ions to the humate samples. The aim of the diploma thesis was to found applicable ion concentration to observation fluorescence quenching and to determine the main fluorophore, which is affected by complexation through the use of synchronous fluorescence spectroscopy.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216498 |
| Date | January 2009 |
| Creators | Hladík, Tomáš |
| Contributors | Pospíšilová, Ľubica, Fasurová, Naděžda |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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