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Stanovení aktivních látek v instantních nápojích určených pro dětskou výživu / Analysis of active substances in instant beverages for child´s nutrition

The aim of presented diploma thesis was to characterize baby beverages for early infant feeding. Theoretical part deals with baby foods and beverages including breast milk. Practical part is focused on analysis of active substances in 21 commercially available fruit and herbal instant granular baby beverages and in 3 packaged tea bags for infant and toddler age. To evaluation of antioxidant properties some group parameters were used - total polyphenols, total flavonoids, total antioxidant activity and anthocyanins. All of them were analysed by using spectrophotometrical methods. For identification and quantification of individual phenolic substances was used RP-HPLC/UV-VIS and HPLC/PDA/LC-MS, identification of individual mono- and disaccharides was performed by HPLC/RI. L-ascorbic acid supplemented to some children's drinks was analysed by HPLC-NH2/UV. Protein analysis was performed by Experion microfluidic electrophoresis system (BioRad) and protein concentration was verified by colorimetric method. In general, the highest content of phenolic compounds was shown in tea bags when compared with instant granular beverages that contain mostly added simple carbohydrates. Using contact microbial tests proved immediately after opening and also during manipulation with tea boxes was verified that analysed baby teas are in agreement with strict hygiene requirements for baby beverages.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216755
Date January 2011
CreatorsJašková, Martina
ContributorsKočí, Radka, Márová, Ivana
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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