Diploma work focuses mainly on the influence of vermicomposting process, which should reduce the incidence of bacteria of family Enterococcus and bacteria of family of Salmonella spp. in the product. As these are among the famous and important human and animal pathogens there was an experiment done to prove this statement.
The experiment took 10 weeks. During the first 14 days (from 9th November 2015) there were samples (pomace of grape vine and apple pomace) taken in the research institute FAPPZ in Červený Újezd. The earthworms were taken form the materials and then taken to SZÚ in Prague with the material, where there was the preparation and analyses of the sample done.
The procedure of sample work was as following. 50g of homogenised material was placed into 48 perforated dishes. The material for the lab experiment was prepared from vermicompost (75%) and from the raw material (25%) so that there was enough nourishment for the earthworms for the duration of the trial. There were certain numbers of microorganisms stated (CPM) in this material. Salmonella reached the values of < 1 CFU/g as the initial substrate. Enterococci in the pomace from the grape vine with earthworms reached the values of 1,1.10 CFU/g, at pomace from the grape vine without earthworms < 750 CFU/g and at apple pomace the value was always < 750 CFU/g. The eathworms were placed upto the half of the glass (2,5g) 6 dishes were chosen from each material, 2 of them served as a check, 2 were inoculated enterococci and 2 with salmonella. There was always one out of 2 dishes placed with earthworms. After the filling there was inoculated of the examined microorganisms. Inoculated variants with enterococci were inoculated of 2.0.107 CFU/g and variants of salmonella by 1.1.108 CFU/g. After inoculated there was sand placed to the dish.
During 8 weeks in frequency of 14 days the chosen samples were taken for analyses. The first analyses was done 23rd November 2025, the 2nd analyses was done 7th December, 3rd analyses was done 21st December 2015 and the 4th analyses was done 4th January 2016. Before the analyses was done the eartworms were taken out of the dish, they were weighted then they vitality was stated.
It was proved there there isn´t any influence of earthworms on the reduction of pathogens according to the experiments. It is impossible to state that there is the influence of eartworms on the process of making compost. The reduction of pathogens was the highest at the family of Salmonella spp. There was obvious reduction at the second week of analyses and in both materials. Bacteria of the family Enterococcus showed slower process of reduction because of higher resistance to temperatures, pH, chemical substances and preparations. The absolute reduction was obvious the during the last week of the experiment.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:256729 |
| Date | January 2016 |
| Creators | Ifková, Sandra |
| Contributors | Hanč, Aleš, Petra, Petra |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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