In humans, multi enzymatic processes are involved in maintaining DNA stability and cellular homeostasis. Cells undergo several episodes to survive and protect itself in daily basis. Accumulation of DNA errors and breaks are repaired by dynamic machinery, such as mismatch repair (MMR), replication-related process. In presented diploma thesis, we report the studied MMR pathway and its involvement in malignancy of epithelial ovarian cancer (EOC). Our working hypothesis postulated that core genes of MMR, such as MLH1 and MSH2 are down-regulated in malignant cells. Cells therefore become incapable to repair accumulating DNA damage, undergo apoptosis or most likely uncontrolled proliferation. Above mentioned genes may also be silenced in cancer patients at transcription, translation or epigenetic levels. Our aims were to clarify and to investigate the importance of MMR based on mRNA transcription, protein stability and promoter hypermethylation on a set of major MMR genes, particularly MLH1, MSH2, PMS1, MLH3, MSH6, MSH3, and PMS2. In our study, we analysed samples from 63 epithelial ovarian cancer patients and 12 non-malignant reference tissues using RT-qPCR, MS-HRM, and Western Blotting methods. Consequently, our results show down-regulation of all MMR genes except for MSH2 (up-regulated) in tumor...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:345028 |
| Date | January 2016 |
| Creators | Burócziová, Monika |
| Contributors | Vodička, Pavel, Schierová, Michaela |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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