The thesis deals with Leishmania development in sand flies focusing on factors that could affect the vectorial part of their lifecycle. First, we examined an effect of temperature on Leishmania development in sand flies since the global warming was suggested to be one of the most important factors affecting dissemination of leishmaniasis. Development of three Leishmania species were studied at two different temperatures (20 and 26řC); L. infantum in natural vectors Lutzomyia longipalpis and Phlebotomus perniciosus, and two closely related Viannia species L. braziliensis and L. peruviana in Lu. longipalpis. Leishmania peruviana thrived well only at the lower temperature tested. At the higher temperature, most of infections were lost between days 2 and 8 post feeding, i.e. time of blood-meal remnants defecation. This suggests that this Andean species is adapted to slower metabolism of vectors living at lower temperatures. On the other hand, L. infantum and L. braziliensis successfully developed in sand flies at both temperatures tested, which might be substantial for their further spread to new areas. Next, an impact of gregarine Psychodiella sergenti on L. tropica in Phlebotomus sergenti was studied. Gregarines are commonly found in sand flies, and in mosquitoes, they can affect development of other...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:350130 |
| Date | January 2015 |
| Creators | Hlaváčová, Jana |
| Contributors | Volf, Petr, Alten, Bülent, Traub-Cseko, Yara Maria |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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