The ability of the ligninolytic fungus Pleurotus ostreatusto degrade 4 pharmaceutical drugs and 5 compounds which are either used during drug manufacturing or are created as by-products was studied. These compounds were detected in groundwater near a drug manufacturing plant. The maximum concentration levels of the selected compounds in tested groundwater samples variedfrom0.23 µg/lto 227.87 µg/l apart from 1 compound which was not detected in any sample. The degradation efficiency of P. ostreatus was examined with individual compounds as well as with the mixture of all 9 compounds. When degrading individual compounds P. ostreatus lowered the initial concentration (10 mg/l) of 5 compounds by 62-100% after 14-day cultivation in malt extract-glucose medium. When degrading the compound mixture P. ostreatus lowered the initial concentration (2 mg/l of each compound) of 5 compounds by 50-100% after 14-day cultivation in malt extract-glucose medium. Acute toxicity tests with Vibrio fischeri suggest the formation of metabolites which are more toxic than the original compounds. The EC50 value for individual compounds during toxicity tests with Vibrio fischeri was 5.45-131.98 mg/l. Keywords:biodegradation, pharmaceuticals, ligninolytic fungi, Pleurotus ostreatus, groundwater, toxicity, Vibrio fischeri
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:353830 |
| Date | January 2015 |
| Creators | Krejčová, Lucie |
| Contributors | Cajthaml, Tomáš, Pivokonský, Martin |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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