This bachelor thesis deals with the development of molecular selection markers for the detection of fertility restorers for CMS Shaan 2A in rape by molecular techniques. The thesis describes molecular techniques such as DNA isolation techniques, polymerase chain reaction and primer design techniques for the detection of fertility restorers. Specific PCR primers for the Pentatricopeptide repeat (PPR) superfamily protein gene and the Tetratricopeptide repeat (TPR) -like superfamily protein gene sequence amplification have been designed and aplicons have been sequenced. On the basis of the sequence differences between plants with fertility restorer fenotype and CMS fenotype the technique for fertility restorer detection was designed.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:376223 |
| Date | January 2018 |
| Creators | KARBANOVÁ, Aneta |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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