The main task of this thesis is to find the ICT activator of a carotenoid spheroidenone in the pigment-protein complex LH1 of Rhodobacter sphaeroides. The ICT state is characterized by a red-shifted positive transient absorption signal around 750 - 800 nm. We hypothesize that the presence of the ICT state is associated with the pigment-protein interaction of a carotenoid keto group and specific amino acid in the protein. To perform the experiment, four mutant complexes with amino acids substitutions, which should result in decreased intensity of the ICT signal due to a weaker or missing pigment-protein interaction, were obtained and measured using femtosecond time resolved pump-probe spectroscopy.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:394544 |
| Date | January 2019 |
| Creators | ŠÍMOVÁ, Ivana |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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