Electron tomography allows visualization of objects in a form of reconstructed 3D virtual volumes with resolution power of electron microscopy. The thesis is focused primarily on biological applications of electron tomography applied on tilt series images acquired in transmission electron microscope at room temperature. Specifically, the interaction of tick-borne encephalitis virus with neural cells and 3D ultrastructure of the central electron-dense part of the flagellum 9 + 1 (Caryophyllaeides fennica) were investigated. Finally, electron tomography was combined and correlated with atomic force microscopy to allow repetitive examination of ultrathin sections on electron microscopy grids.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:394991 |
| Date | January 2019 |
| Creators | BÍLÝ, Tomáš |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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