The theoretical overview summarize the characteristics of algae and their primary and secondary metabolism. It also deals with inhibitors of ascorbic acid, glutathione and polyphenolic compounds synthesis. The practical part studies the influence of selected inhibitors (BSO, Lyc, AIP) and CdCl2 on the secondary metabolites production and biomass growth in freshwater microalgae Scenedesmus quadricauda. The content of secondary metabolites (eg. caffeic acid, chlorogenic acid, salicylic acid, cinnamic acid, p-coumaric acid) was detected by HPLC-MS. Furthermore, the total antioxidant capacity, the total polyphenol content and the total flavonoid content was determined spectrophotometrically. All the inhibitors used, reduced S. quadricauda growth in comparison with control samples. The most pronounced inhibitory effect was observed in the samples treated with Lyc + Cd after 7 days of experiment. All the treated samples responded to the stress factors by altering metabolic pathways and inhibiting growth. As a result, there were changes in the composition and the amount of selected secondary metabolites versus the control samples. At the same time, in most cases, the biosynthesis of polyphenolic and flavonoid substances has been stimulated. The oxidative reduction equilibrium and the induction of oxidative stress were probably impaired.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427102 |
| Date | January 2018 |
| Creators | Rankić, Ivan |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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