Agricultural production is negatively affected by stress factors like drought or rising ambient temperature. Genetic engineering and production of specific GMO seems to be very promising in protection of the crop yield however these are strictly limited for the use in the field. For this reason alternative approaches like application of growth regulators are in the focus of recent research. INCYDE is a new potent growth regulator discovered as inhibitor of cytokinin degradation. In this Diploma thesis we show that high levels of INCYDE could also activate AHK4, cytokinin receptor, and act like cytokinins. Comparison of trans-zeatin and INCYDE showed that application of these regulators results in spatial-specific pattern in activation of the cytokinin signaling. trans-zeatin and INCYDE also induce different changes in proteom mainly in proteasome pathway, gluconeogenesis and fatty acids metabolism. Application of INCYDE simultaneously with inducing drought stress revealed that INCYDE could decrease sensitivity to this stressor. This feature seems to be very perspective for its utilization in the agriculture.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:430162 |
| Date | January 2017 |
| Creators | Koukalová, Vladěna |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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