This thesis deals with selection and verification of suitable mitochondrial markers for species identification of Cepaea molluscs. Optimisation of genomic DNA extraction method, PCR amplification using the 12S and 16S mitochondrial markers and automated sequencing of PCR products were performed. Data was evaluated using SeqScape Software v2.7, Sequence Scanner Software v1.0, MEGA7 programs and BLAST database. The aim of this thesis is to determinate whether the mitochondrial markers 12S and 16S are suitable for DNA barcoding of Cepaea.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:430583 |
| Date | January 2017 |
| Creators | Dratvová, Lenka |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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